Korean J Physiol Pharmacol.  2015 Mar;19(2):119-124. 10.4196/kjpp.2015.19.2.119.

Effects on G2/M Phase Cell Cycle Distribution and Aneuploidy Formation of Exposure to a 60 Hz Electromagnetic Field in Combination with Ionizing Radiation or Hydrogen Peroxide in L132 Nontumorigenic Human Lung Epithelial Cells

• Jin H
• Yoon HE
• Lee JS
• Kim JK
• Myung SH
• Lee YS

• KMID: 2285563
• DOI: http://doi.org/10.4196/kjpp.2015.19.2.119

Abstract

The aim of the present study was to assess whether exposure to the combination of an extremely low frequency magnetic field (ELF-MF; 60 Hz, 1 mT or 2 mT) with a stress factor, such as ionizing radiation (IR) or H2O2, results in genomic instability in non-tumorigenic human lung epithelial L132 cells. To this end, the percentages of G2/M-arrested cells and aneuploid cells were examined. Exposure to 0.5 Gy IR or 0.05 mM H2O2 for 9 h resulted in the highest levels of aneuploidy; however, no cells were observed in the subG1 phase, which indicated the absence of apoptotic cell death. Exposure to an ELF-MF alone (1 mT or 2 mT) did not affect the percentages of G2/M-arrested cells, aneuploid cells, or the populations of cells in the subG1 phase. Moreover, when cells were exposed to a 1 mT or 2 mT ELF-MF in combination with IR (0.5 Gy) or H2O2 (0.05 mM), the ELF-MF did not further increase the percentages of G2/M-arrested cells or aneuploid cells. These results suggest that ELF-MFs alone do not induce either G2/M arrest or aneuploidy, even when administered in combination with different stressors.

Keyword

Aneuploidy; Extremely low frequency; G2/M phase; H2O2; Ionizing radiation

MeSH Terms

Aneuploidy*
Cell Cycle*
Cell Death
Electromagnetic Fields*
Epithelial Cells*
Genomic Instability
Humans
Hydrogen Peroxide*
Lung*
Magnetic Fields
Radiation, Ionizing*
Hydrogen Peroxide

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