Korean J Physiol Pharmacol.  2012 Dec;16(6):455-462. 10.4196/kjpp.2012.16.6.455.

Protective Effect of ECQ on Rat Reflux Esophagitis Model

• Jang HS
• Han JH
• Jeong JY
• Sohn UD

• KMID: 2285455
• DOI: http://doi.org/10.4196/kjpp.2012.16.6.455

Abstract

This study was designed to determine the protective effect of Rumex Aquaticus Herba extracts containing quercetin-3-beta-D-glucuronopyranoside (ECQ) on experimental reflux esophagitis. Reflux esophagitis was induced by surgical procedure. The rats were divided into seven groups, namely normal group, control group, ECQ (1, 3, 10, 30 mg/kg) group and omeprazole (30 mg/kg) group. ECQ and omeprazole groups received intraduodenal administration. The Rats were starved for 24 hours before the experiments, but were freely allowed to drink water. ECQ group attenuated the gross esophagitis significantly compared to that treated with omeprazole in a dose-dependent manner. ECQ decreased the volume of gastric juice and increased the gastric pH, which are similar to those of omeprazole group. In addition, ECQ inhibited the acid output effectively in reflux esophagitis. Significantly increased amounts of malondialdehyde (MDA), myeloperoxidase (MPO) activity and the mucosal depletion of reduced glutathione (GSH) were observed in the reflux esophagitis. ECQ administration attenuated the decrement of the GSH levels and affected the MDA levels and MPO activity. These results suggest that the ECQ has a protective effect which may be attributed to its multiple effects including anti-secretory, anti-oxidative and anti-inflammatory actions on reflux esophagitis in rats.

Keyword

Antioxidant; Extracts; Rats; Reflux esophagitis; Rumex Aquaticus Herba

MeSH Terms

Animals
Control Groups
Esophagitis
Esophagitis, Peptic
Gastric Juice
Glutathione
Hydrogen-Ion Concentration
Malondialdehyde
Omeprazole
Peroxidase
Rats
Rumex
Water
Glutathione
Malondialdehyde
Omeprazole
Peroxidase
Water

Figure

• 

  Fig. 1 The esophagus lesions induced by a surgical procedure and the effects of treatment with ECQ and omeprazole. (A) Normal. (B) Reflux esophagitis induced by a surgical procedure. (C~F) Treatment with ECQ 1, 3, 10, 30 mg/kg after surgical procedure. (G) Treatment with omeprazole 30 mg/kg after surgical procedure.

• 

  Fig. 2 The effects of ECQ and omeprazole on the esophagus lesions induced by a surgical procedure in rats. The animals were sacrificed after 24 hours on starting the treatment, and the esophagus was examined. The esophagus lesions were decreased in ECQ dose-dependent manner. Data are expressed as means±SEM, n=5. Student's t-test; *, Significant at p<0.01 vs Normal group. #, Significant at p<0.05 vs Control group.

• 

  Fig. 3 The effects of ECQ and omeprazole on the gastric volume in reflux esophagitis model induced by a surgical procedure. The gastric volume in the control group with reflux esophagitis was increased compared to the normal group. The increased gastric volumes in reflux esophagitis models were significantly decreased after administration of ECQ 30 mg/kg. Data are expressed as means±SEM, n=5. Student's t-test; *, Significant at p<0.05 vs Normal group. #, Significant at p<0.05 vs Control group.

• 

  Fig. 4 The effects of ECQ and omeprazole on the pH in rat reflux esophagitis induced by a surgical procedure. The pH in the control group with reflux esophagitis was decreased compared to the normal group. The pH in rats with reflux esophagitis was significantly increased in ECQ dose-dependent manner after treatment with ECQ. Data are expressed as means±SEM, n=5. Student's t-test; *, Significant at p<0.05 vs Normal group. #, Significant at p<0.05 vs Control group.

• 

  Fig. 5 The effects of ECQ and omeprazole on acid output in rats with reflux esophagitis induced by surgical procedure. The acid output in the control group with reflux esophagitis was increased compared to the normal group. The acid output in rats with reflux esophagitis was significantly decreased in ECQ dose-dependent manner. Data are expressed as means±SEM, n=5. Student's t-test; *, Significant at p<0.05 vs Normal group. #, Significant at p<0.05 vs Control group.

• 

  Fig. 6 The effects of ECQ and omeprazole on myeloperoxidase (MPO) activity in rats with reflux esophagitis induced by a surgical procedure. The ECQ (1, 3, 10, 30 mg/kg) and omeprazole (30 mg/kg) were treated after the surgical procedure. The increased MPO activity in the reflux esophagitis models was significantly decreased in ECQ dose-dependent manner. Data are expressed as means±SEM, n=5. Student's t-test; *, Significant at p<0.01 vs Normal group. #, Significant at p<0.05 vs Control group. ##, Significant at p<0.01 vs Control group.

• 

  Fig. 7 The effects of ECQ and omeprazole on malondialdehyde (MDA) levels in rats with reflux esophagitis induced by a surgical procedure. The ECQ (1, 3, 10, 30 mg/kg) and omeprazole (30 mg/kg) were treated after the surgical procedure. The increased MDA levels in the reflux esophagitis models were significantly decreased in ECQ dose-dependent manner. Data are expressed as means±SEM, n=5. Student's t-test; *, Significant at p<0.05 vs Normal group. #, Significant at p<0.01 vs Control group.

• 

  Fig. 8 The effects of ECQ and omeprazole on glutathione (GSH) levels in rats with reflux esophagitis induced by a surgical procedure. The ECQ (1, 3, 10, 30 mg/kg) and omeprazole (30 mg/kg) were treated after the surgical procedure. The decreased GSH levels in the reflux esophagitis models were significantly increased after administration of ECQ 30 mg/kg. Data are expressed as means±SEM, n=5. Student's t-test; *, Significant at p<0.05 vs Normal group. #, Significant at p<0.05 vs Control group.

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