Korean J Physiol Pharmacol.  2008 Jun;12(3):111-115. 10.4196/kjpp.2008.12.3.111.

(-)-Epigallocatechin Gallate Inhibits the Pacemaker Activity of Interstitial Cells of Cajal of Mouse Small Intestine

Affiliations
  • 1Department of Rehabilitation, College of Medicine, Chosun University, Gwangju 501-759, Korea.
  • 2Department of Radiology, Gacheon University Gil Medical Center, Incheon 405-760, Korea.
  • 3Department of Psychiatry, College of Medicine, Chosun University, Gwangju 501-759, Korea.
  • 4Department of Physiology, College of Medicine, Chosun University, Gwangju 501-759, Korea. jyjun@chosun.ac.kr

Abstract

The effects of (-)-epigallocatechin gallate (EGCG) on pacemaker activities of cultured interstitial cells of Cajal (ICC) from murine small intestine were investigated using whole-cell patch-clamp technique at 30degrees C and Ca2+ image analysis. ICC generated spontaneous pacemaker currents at a holding potential of -70 mV. The treatment of ICC with EGCG resulted in a dose-dependent decrease in the frequency and amplitude of pacemaker currents. SQ-22536, an adenylate cyclase inhibitor, and ODQ, a guanylate cyclase inhibitor, did not inhibit the effects of EGCG. EGCG-induced effects on pacemaker currents were not inhibited by glibenclamide, an ATP-sensitive K+ channel blocker and TEA, a Ca2+-activated K+ channel blocker. Also, we found that EGCG inhibited the spontaneous [Ca2+]i oscillations in cultured ICC. In conclusion, EGCG inhibited the pacemaker activity of ICC and reduced [Ca2+]i oscillations by cAMP-, cGMP-, ATP-sensitive K+channel-independent manner.

Keyword

(-)-epigallocatechin gallate (EGCG); Interstitial cells of Cajal (ICC); Pacemaker currents; Intestinal motility
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