Korean J Physiol Pharmacol.  2003 Aug;7(4):247-250.

A Simple and Sensitive Assay for Cefepime in Human Plasma Using High Performance Liquid Chromatography

• Kim YS
• Yim DS
• Lee DG
• Lee SB

Abstract

A simple and sensitive assay method was developed for cefepime in human plasma using high performance liquid chromatography (HPLC). Cefepime and cefadroxil (the internal standard) were extracted from heparinized human plasma by simple deproteination with perchloric acid. The extract was injected into an Atlantis dC18 column (250 X 4.6 mm; particle size 5micrometer, Waters) and the column was eluted with methanol and 0.01 M dihydrogen phosphate at pH 3.0 (15 : 85 v : v) as a mobile phase at a flow rate of 0.7 mL/min. Linearity was confirmed for the range 0.25 to 200 micro L/mL and the limit of quantitation was 0.25 micro L/mL. The retention times were 10.2 min and 13.4 min for cefepime and cefadroxil, respectively. This method was successfully applied to a pharmacokinetic study of cefepime in plasma from bone marrow transplant patients.

Keyword

Cefepime; Cefadroxil; HPLC

MeSH Terms

Bone Marrow
Cefadroxil
Chromatography, High Pressure Liquid
Chromatography, Liquid*
Heparin
Humans*
Hydrogen-Ion Concentration
Methanol
Particle Size
Plasma*
Cefadroxil
Heparin
Methanol