Korean J Physiol Pharmacol.  2001 Apr;5(2):157-163.

Block of ATP-sensitive K+ channels expressed in Xenopus oocytes by dimethyl sulfoxide

• Park JB
• Chae SW

Abstract

The effects of dimethyl sulfoxide (DMSO) were studied in two groups of Xenopus oocytes, one expressing ATP sensitive K+ (KATP) channel comprised of sulfonylurea receptor SUR1 and inwardly rectifying K+ channel subunit Kir6.2, and the other expressing renal KATP channel ROMK2. At concentrations of 0.3~10% (vol/vol) DMSO inhibited whole cell Kir6.2/SUR1 currents elicited by bath application of sodium azide (3 mM) in a concentration-dependent manner. The inhibition constant and Hill coefficient were 2.93% and 1.62, respectively. ROMK2 currents, however, was not affected significantly by DMSO. The results support the idea that DMSO inhibits KATP channel expressed in Xenopus oocyte through a protein-specific mechanism(s) that remains to be further elucidated.

Keyword

Dimethyl sulfoxide; ATP-sensitive K channel; ROMK2; Xenopus oocyte

MeSH Terms

Adenosine Triphosphate
Baths
Dimethyl Sulfoxide*
Oocytes*
Sodium Azide
Xenopus*
Adenosine Triphosphate
Dimethyl Sulfoxide
Sodium Azide