Korean J Physiol Pharmacol.  1999 Jun;3(3):251-261.

Effects of carnosine and related compounds on monosaccharide autoxidation and H2O2 formation

Affiliations
  • 1Laboratory of Veterinary Public Health, College of Veterinary Medicine, Seoul National University, Suwon, 441-744 South Korea.
  • 2Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Suwon, 441-744 South Korea.
  • 3Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Suwon 441-744, Korea.
  • 4Department of Veterinary Physiology, College of Veterinary Medicine, Chungbuk National University, Cheongiu 361-763, Korea.

Abstract

The effects of carnosine and related compounds (CRCs) including anserine, homocarnosine, histidine, and beta-alanine on monosaccharide autoxidation and H2O2 formation were investigated. The incubation of CRCs with D-glucose, D-glucosamine, and D, L-glyceraldehyde at 37degreeC increased the absorption maxima at 285 nm, 273 nm, and 290 ~ 330 nm, respectively. D, L-glyceraldehyde was the most reactive sugar with CRCs. The presence of copper strongly stimulated the reaction of carnosine and anserine with D-glucose or D-glucosamine. Carnosine and anserine stimulated H2O2 formation from D-glucose autoxidation in a dose-dependent manner in the presence of 10 muM Cu (II). The presence of human serum albumin (HSA) decreased their effect on H2O2 formation. Carnosine and anserine has a biphasic effect on alpha-ketoaldehyde formation from glucose autoxidation. CRCs inhibited glycation of HSA as determined by hydroxymethyl furfural, lysine residue with free epsilon-amino group, and fructosamine assay. These
results
suggest that CRCs may be protective against diabetic complications by reacting with sugars and protecting glycation of protein.

Keyword

Carnosine and related compounds; Glucose oxidation; alpha-ketoaldehyde; Free radicals; Glycation; Antioxidant

MeSH Terms

Absorption
Anserine
beta-Alanine
Carbohydrates
Carnosine*
Copper
Diabetes Complications
Free Radicals
Fructosamine
Furaldehyde
Glucose
Histidine
Humans
Lysine
Serum Albumin
Anserine
Carbohydrates
Carnosine
Copper
Free Radicals
Fructosamine
Furaldehyde
Glucose
Histidine
Lysine
Serum Albumin
beta-Alanine
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