Korean J Physiol Pharmacol.  1998 Aug;2(4):455-460.

Altered renal nitric oxide system in experimental hypertensive rats

• Yang ES
• Kang J
• Lee DG

Abstract

The present study was aimed at investigating whether the development of hypertension is related with an altered expression of nitric oxide synthases (NOS) in the kidney. By Western blot analysis, the expression of bNOS and ecNOS isoforms was determined in the kidney of deoxycorticosterone acetate (DOCA)-salt and two-kidney, one clip (2K1C) rats. In DOCA-salt hypertension, the expression of both bNOS and ecNOS was decreased, along with tissue contents of nitrites. In 2K1C hypertension, the nitrite content of the clipped kidney was decreased along with ecNOS levels, whereas neither the nitrite content nor the expression of NOS isoforms was significantly altered in the contralateral non-clipped kidney. These results suggest that the development of hypertension is associated with an altered renal expression of NOS and nitric oxide generation in DOCA-salt and 2K1C rats.

Keyword

Kidney; Nitric oxide synthases; Nitrite contents; Deoxycorticosterone acetate-salt hypertension; Two-kidney; One clip hypertension

MeSH Terms

Animals
Blotting, Western
Desoxycorticosterone
Hypertension
Kidney
Nitric Oxide*
Nitrites
Protein Isoforms
Rats*
Desoxycorticosterone
Nitric Oxide
Nitrites
Protein Isoforms