Korean J Physiol Pharmacol.  1998 Aug;2(4):419-425.

Effects of exogenous ATP on calcium mobilization and cell proliferation in C6 glioma cell

Affiliations
  • 1Department of Pharmacology, Catholic University Medical College, 505 Banpo-dong, Seocho-gu, Seoul 137-701 South Korea.

Abstract

To clarify the effect of extracellular ATP in cultured C6 glioma cells, ATP-induced cytosolic free calcium ((Ca2+)i) mobilization and cell proliferation were investigated. ATP-induced (Ca2+)i increased in a dose-dependent manner (10-7 M apprx 10-3 M). ATP-induced (Ca2+)i increases were slightly slowed in extracellular calcium-free conditions especially in sustained phase. ATP-induced (Ca2+)i increment was also inhibited by the pretreatment of U73122, a phospholipase C (PLC) inhibitor, in a time-dependent manner. Suramin, a putative P2Y receptor antagonist, dose-dependently weakened ATP-induced (Ca2+)i mobilization. Significant increases in cell proliferation were observed at 2, 3, and 4 days after ATP was added. Stimulated cell proliferation was also observed with adenosine at days 2 and 3. This cell proliferation was significantly inhibited by the treatment with suramin. Ionomycin also stimulated cell proliferation in a concentration-dependent manner. In
conclusion
, we suggest that extracellular ATP stimulates C6 glioma cell proliferation via intracellular free calcium mobilization mediated by purinoceptor.

Keyword

ATP, Purinoceptor; C6 glioma cell; Cytosolic free calcium; Cell proliferation

MeSH Terms

Adenosine
Adenosine Triphosphate*
Calcium*
Cell Proliferation*
Cytosol
Glioma*
Ionomycin
Receptors, Purinergic
Suramin
Type C Phospholipases
Adenosine
Adenosine Triphosphate
Calcium
Ionomycin
Receptors, Purinergic
Suramin
Type C Phospholipases
Full Text Links
  • KJPP
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr