Korean J Otorhinolaryngol-Head Neck Surg.
2008 Jan;51(1):28-32.
Isolation and Culture of Adult Neural Stem Cells from Guinea Pig Tympanic Membrane
- Affiliations
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- 1Department of Otorhinolaryngology, The Catholic University of Korea College of Medicine, Seoul, Korea. swyeo@catholic.ac.kr
Abstract
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BACKGROUND AND OBJECTIVES: Tympanic membrane perforation is an important clinical problem found in various populations of the world. In large number of cases, acute traumatic perforations heal spontaneously, and in the healing process, stem cells appear to play an important role. However, no studies have been reported regarding somatic stem cells in the tympanic membrane. Herein, we tried to show that guinea pig's tympanic membrane contains cells that display the characteristic features of stem cells.
MATERIALS AND METHOD: The tympanic membrane was obtained from the guinea pig. The cells were cultured in a medium with epidermal growth factor (EGF) and fibroblast growth factor (FGF). Proliferating cells were checked with stem cell markers, bromodeoxyuridine (BrdU) and nestin. Differentiated cells from stem cells are checked with betaIII tubulin and S-100.
RESULTS
We observed that some of the cultured cells from the tympanic membrane were stained with both stem cell markers, BrdU and nestin. And we observed that these cells differentiated into neuron and gilal cells, which expressed betaIII tubulin and S-100, respectively.
CONCLUSION
These results suggest that the tympanic membrane of guinea pigs may have neural stem cells. Further study is needed for finding the origin of stem cells.
Keyword
MeSH Terms
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Adult
Adult Stem Cells
Animals
Bromodeoxyuridine
Cells, Cultured
Epidermal Growth Factor
Fibroblast Growth Factors
Guinea
Guinea Pigs
Humans
Intermediate Filament Proteins
Nerve Tissue Proteins
Neural Stem Cells
Neurons
Stem Cells
Tubulin
Tympanic Membrane
Tympanic Membrane Perforation
Bromodeoxyuridine
Epidermal Growth Factor
Fibroblast Growth Factors
Intermediate Filament Proteins
Nerve Tissue Proteins
Tubulin
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