Korean J Otolaryngol-Head Neck Surg.  2003 Oct;46(10):822-826.

The Effect of Substance P on Osteoclastogenesis and Osteoclastic Bone Resorption in vitro

• Sohn SJ

Abstract

BACKGROUND AND OBJECTIVES
Osteoclasts are the principal cell of bone resorption playing a major role in focal bone erosion associated with cholesteatoma. This study was conducted in order to investigate direct effect of substance P (SP) on osteoclastogenesis and osteoclastic bone resorption in vitro. MATERIALS AND METHOD: SP dose response was measured in receptor activator for NF-kappaB ligand (RANKL)-induced mouse osteoclast culture and osteoclastic bone resorption assay. RT-PCR was performed for the expression of neurokinin(NK) receptor mRNAs in osteoclasts. RESULTS: Treatment with SP (100 nM and 1000 nM) significantly increased osteoclastogenesis. SP (0.1 nM and 1 nM) significantly increased resorption surface area on dentin slices by osteoclasts. Cultured osteoclasts expressed NK-2 receptor mRNA. CONCLUSION: SP has a direct upregulatory effect on osteoclastogenesis and osteoclastic bone resorption that is mediated possibly by NK-2 receptor.

Keyword

Osteoclasts; Substance P; Receptors; Neurokinin-2; Bone resorption

MeSH Terms

Animals
Bone Resorption*
Cholesteatoma
Dentin
Mice
NF-kappa B
Osteoclasts*
Receptors, Neurokinin-2
RNA, Messenger
Substance P*
NF-kappa B
RNA, Messenger
Receptors, Neurokinin-2
Substance P