Korean J Otolaryngol-Head Neck Surg.  2003 Feb;46(2):110-114.

The Effects of Intranasal Instillation of Stem Cell Factor in Mice with Experimentally-Induced Allergic Rhinitis

Affiliations
  • 1Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul, Korea. ygmin@plaza.snu.ac.kr

Abstract

BACKGROUND AND OBJECTIVES
The pathogenesis of allergic rhinitis has not been fully understood. The role of stem cell factor (SCF) in allergic airway inflammation has recently been identified. This study aimed to investigate the effect of intranasal instillation of SCF in mice with experimentally-induced allergic rhinitis. MATERIALS AND METHOD: Male BALB-c mice were intraperitonially sensitized to ovalbumin mixed with aluminum hydroxide and were challenged with aerosols of ovalbumin. Recombinant murine SCF was directly instilled to both nasal cavities. Sneezing and nose-rubbing act of the mice were checked by symptom scores. Nasal lavage fluid (NLF) was collected and histamine levels in the NLF were measured. The eosinophils in the inferior turbinate of the mice were counted at the coronal section of the skull. RESULTS: The symptom scores checked 1 hour after intranasal instillation of 50 nM SCF and 500 nM SCF were 29+/-8 (mean+/-S.D.) and 31+/-10, respectively. They were significantly different from those of the control group (18+/-5). Histamine levels of NLF collected 1 hour after intranasal instillation of 500 nM SCF were 3.62 1.8 nM and were significantly different from those of the control group (1.31+/-0.4 nM). The number of eosinophils in the inferior turbinate of mice counted 24 hours after intranasal instillation of 500 nM SCF was 17.6+/-3.2 and was significantly different from those of the control group (11.2+/-3.5). CONCLUSION: Stem cell factor may play an important role in the pathogenesis of allergic rhinitis.

Keyword

Stem cell factor; Allergic rhinitis; Ovalbumin; Histamine; Eosinophil
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