Korean J Otolaryngol-Head Neck Surg.  2001 Sep;44(9):930-935.

Effects of Dexamethasone on RANTES Expression of Nasal Fibroblast

• Jin YW
• Cho JS
• Kim H
• Kim CD
• Jin SY
• Cha CI

Abstract

BACKGROUND AND OBJECTIVES
Fibroblasts play an indirectly augmenting effector role in allergic inflammatory response by releasing different proinflammatory cytokines, including RANTES, GM-CSF, IL-8 after stimulation by other inflammatory cytokines such as IFN-gamma, TNF-alpha. The aim of this study was to investigate expression of RANTES in allergic and non-allergic nasal fibroblasts after stimulation with IFN-gamma and TNF-alpha, and to study the effect of dexamethasone on the RANTES expression of nasal fibroblast cell.
MATERIALS AND METHODS
Using the 3rd passage of fibroblasts taken from the inferior turbinates of allergic and non-allergic patients, we evaluated the RANTES expression of fibroblasts after the IFN-gamma, TNF-alpha stimulation in the presence or in the absence of dexamethasone by ELISA.
RESULTS
The expression of RANTES in allergic nasal fibroblasts stimulated by cytokines was stronger than in non-allergic nasal fibroblasts stimulated by cytokines. And dexamethasone suppressed the RANTES expression in allergic nasal fibroblasts stimulated by IFN-gamma. However, dexamethasone did not affect the RANTES expression in allergic fibroblasts stimulated by TNF-alpha and non-allergic fibroblasts stimulated by IFN-gamma and TNF-alpha.
CONCLUSION
This study shows different responses of the RANTES production in nasal fibroblasts to dexamethasone, perhaps reflecting heterogeneity of nasal fibroblasts.

Keyword

Fibroblast; RANTES; Dexamethasone

MeSH Terms

Chemokine CCL5*
Cytokines
Dexamethasone*
Enzyme-Linked Immunosorbent Assay
Fibroblasts*
Granulocyte-Macrophage Colony-Stimulating Factor
Humans
Interleukin-8
Population Characteristics
Tumor Necrosis Factor-alpha
Turbinates
Chemokine CCL5
Cytokines
Dexamethasone
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-8
Tumor Necrosis Factor-alpha