Korean J Obstet Gynecol.  2009 Apr;52(4):447-455.

Characterization and differentiation into adipocytes of mesenchymal stem cells (MSCs) from human adipose tissue and amniotic fluid

• Kim HK
• Im EH
• Park H
• Cho JA
• Yang DY
• Kim KH
• Lee KW

Abstract

OBJECTIVE
Mesenchymal stem cells (MSCs) are potentially very useful for regenerative and reparative medicine as well as therapeutic possibilities. The aim of this study is to examine the ability of ADSCs and AFSCs to be phenotypically and functionally differentiated into adipocyte and to determine the appropriate stem cell source and conditions for efficient adipocyte regeneration.
METHODS
Adipogenic differentiation was induced by culturing confluent ADSCs and AFSCs in adipogenic medium for 2~4 weeks. During the differentiation inducing period, we evaluated the successful adipogenesis by performing immunocytochemistry and RT-PCR to detect the lipid producibility and several adipogenic gene expressions including lipoprotein lipase (LPL), peroxisome proliferator-activated receptor gamma2 (PPAR gamma2) and adiponectin.
RESULTS
ADSCs and AFSCs are expanded easily in vitro and exhibited a fibroblast-like morphology as previously known in MSCs from bone marrow and a commercial source. Flow cytometric analysis showed that ADSCs and AFSCs expressed several CD marker antigens similar to those observed on bone marrow-derived MSCs. Adipogenic induction of ADSCs and AFSCs resulted in the extended cell morphology, intracellular staining of an established lipid dye Oil Red O, and expression of adipocyte-specific genes.
CONCLUSION
Both ADSCs and AFSCs successfully differentiate in vitro into adipogenic cells in the presence of the lineage-specific induction factors although ADSC showed the greater capability. Therefore, the results suggest that ADSCs and AFSCs may be an excellent choice for many future tissue engineering strategies and cell-based therapies.

Keyword

Stem cells; Adipose-derived stem cells (ADSCs); Amniotic fluid-derived stem cells (AFSCs); Differentiation; Adipocyte

MeSH Terms

Adipocytes
Adipogenesis
Adipose Tissue
Amniotic Fluid
Antigens, Differentiation
Azo Compounds
Bone Marrow
Female
Gene Expression
Humans
Immunohistochemistry
Lipoprotein Lipase
Mesenchymal Stromal Cells
Peroxisomes
Stem Cells
Tissue Engineering
Antigens, Differentiation
Azo Compounds
Lipoprotein Lipase