Korean J Obstet Gynecol.
2002 Dec;45(12):2140-2145.
Expression of Steroidogenic Acute regulatory (StAR) Protein during Oocyte Apoptosis in the Gonadotropin-Stimulated Human Ovary
- Affiliations
-
- 1Department of Obstetrics and Gynecology, Yonsei University, College of Medicine, Korea.
- 2Department of Life Sciences, Hanyang University, College of Natural Sciences, Korea.
- 3Department of Obstetrics and Gynecology, Ulji University, College of Medicine, Korea.
- 4Infertility Clinic, Seoul Women's Hospital, Korea.
Abstract
OBJECTIVE
To determine the distribution and expression of steroid acute regulatory (StAR) protein in human oocyte and embryo in relation to apoptosis.
METHODS
Immuno-labelling and confocal microscopy were applied to examine the localization of StAR protein in human oocytes and embryos. Western blot analysis was also used for qualitative and quantitative assessment of StAR protein expression.
RESULTS
There were lipid droplet accumulation in fragmented human oocytes and embryos. StAR protein (30 kDa) expression was detected in human oocytes and embryos. The level of StAR protein expression was lower in the fragmented group than the normal group.
CONCLUSION
The present study provides evidence for involvement of StAR protein in the apoptosis of fragmented oocytes and embryos during in vitro fertilization (IVF) program as well as in the normal development of human oocytes and embryos.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Detection of the Steroidogenic Acute Regulatory(StAR) Protein in Human Testicular Tissue
- A Molecular Genetic Study with EcoRII Restriction Enzyme on the Steroidogenic Acute Regulatory Protein (StAR) Gene
- Fancd2os Reduces Testosterone Production by Inhibiting Steroidogenic Enzymes and Promoting Cellular Apoptosis in Murine Testicular Leydig Cells
- Molecular mechanisms of luteinization
- Congenital lipoid adrenal hyperplasia
