The Effect of Coculture with Human Amnion cell on Growth, Development, Implantation in Mouse Embryos

Choi, Won Jun; Shin, Jeong Gyu; Lee, Soon Ae; Lee, Jong Hak; Paik, Won Young

Abstract

OBJECTIVE
This study was performed to investigate the influence of coculture with human amnion cell on growth, development, implantation in mouse embryos.
METHODS
Two-cell and Eight-cell stage mouse embryos were cocultured for 96 hrs with amnion cell. Embryos not treated with amnion were served control. The percentages of embryos which developed to the expanded, hatched blastocyst stage and in vitro implantation at 24hrs, 48hrs, 72hrs were determined.
RESULTS
The percentages of fully expanded murine blastocysts in coculture with amnion cell were not significantly different from control. The percentages of hatched blastocysts were significantly higher in coculture group at two-cell stage (71.6%) compared to control (47.0%; p<0.05). The percentages of hatched blastocysts were significantly higher in coculture group at eight-cell stage (74.0%) compared to control (56.1%; p<0.05). In two-cell stage embryos, the percentages of implanted blastocyst in vitro were significantly higher following coculture with amnion cell (31.6%) compared to control (13.7%).
CONCLUSION
Amnion cell coculture may have a stimulatory role in embryonic development, implantation compared to control. The coculture condition which induces the best effect on in vitro growth and development might be the mimics of the physiologic natural condition, just like the coculture with the tubal epithelium.