Korean J Nutr.  2005 Sep;38(7):503-511.

A Study on the Effects of Anticarcinogenic Activity of Chondria Crassicaulis

• Jeon KH
• Shin MO
• Bae SJ

Abstract

In this study, we investigated the biological activity of Chondria crassicaulis (CC) on the human cancer cells. CC was extracted with methanol and further fractionated into four diffferent types: hexane (CCMH), methanol (CCMM), butanol (CCMB), and aqueous (CCMA) partition layers. We determined the cytotoxic effect of these layers on human cancer cells by MTT assay. Among various partition layers of CC, the CCMM and CCMB showed the strong cytotoxic effects at 150 micrometer/ml which resulted 98.91%, 92.96% on HeLa cell lines and 95.47%, 77.05% on MCF-7 cell lines. And, the anti-proliferative effect of CC was accompanied by a marked inhibition of cyclooxygenase (COX-2), Caspase-3 and IAP (cIAP-1, cIAP-2 and XIAP) protein and concomitant induction of p53, p21 and Survivin protein. However, CC did not affect the level of Bax, Bcl-2 and Bcl-XL protein. Also, we observed quinone reductase (QR) induced effects in all fraction layers of CC on HepG2 cells. The QR induced effects of the CCMH and CCMM on HepG2 cells at 120 micrometer/mL concentration indicated 3.73 and 2.45 with the control value of 1.0. Although further studies are needed, the present work suggests that CC may be a chemopreventive agent for the treatment of human cancer cells.

Keyword

cytotoxicity; quinone reductase; Chondria crassicaulis (CC)

MeSH Terms

bcl-X Protein
Caspase 3
HeLa Cells
Hep G2 Cells
Humans
MCF-7 Cells
Methanol
NAD(P)H Dehydrogenase (Quinone)
Prostaglandin-Endoperoxide Synthases
Caspase 3
Methanol
NAD(P)H Dehydrogenase (Quinone)
Prostaglandin-Endoperoxide Synthases
bcl-X Protein