Ann Rehabil Med.  2014 Feb;38(1):1-5. 10.5535/arm.2014.38.1.1.

The Insertion/Deletion Polymorphism of Angiotensin I Converting Enzyme Gene is Associated With Ossification of the Posterior Longitudinal Ligament in the Korean Population

• Kim DH
• Yun DH
• Kim HS
• Min SK
• Yoo SD
• Lee KH
• Kim KT
• Jo DJ
• Kim SK
• Chung JH
• Ban JY
• Lee SY

• KMID: 2266533
• DOI: http://doi.org/10.5535/arm.2014.38.1.1

Abstract

OBJECTIVE
To determine whether ACE insertion/deletion (I/D) polymorphism is associated with the ossification of the posterior longitudinal ligament (OPLL) of the spine in the Korean population.
METHODS
A case-control study was conducted to investigate the association between I/D polymorphism of the angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 (ACE) gene and OPLL. The 95 OPLL patients and 274 control subjects were recruited. Polymerase chain reaction for the genotyping of ACE I/D polymorphism was performed. The difference between the OPLL patients and the control subjects was compared using the contingency chi2 test and the logistic regression analysis. For statistical analysis, SPSS, SNPStats, SNPAnalyzer, and Helixtree programs were used.
RESULTS
The genotype and allele frequencies of ACE I/D polymorphism showed significant differences between the OPLL patients and the control subjects (genotype, p<0.001; allele, p=0.009). The frequencies of D/D genotype and D allele in the OPLL group were higher than those in the control group. In logistic regression analysis, ACE I/D polymorphism was associated with OPLL (dominant model; p=0.002; odd ratio, 2.20; 95% confidence interval, 1.33-3.65).
CONCLUSION
These results suggest that the deletion polymorphism of the ACE gene may be a risk factor for the development of OPLL in the Korean population.

Keyword

Ossification of the posterior longitudinal ligament; Angiotension converting enzyme; Genetic polymorphism; Genetic association analysis

MeSH Terms

Alleles
Angiotensin I*
Angiotensins*
Case-Control Studies
Gene Frequency
Genotype
Humans
Logistic Models
Longitudinal Ligaments*
Peptidyl-Dipeptidase A*
Polymerase Chain Reaction
Polymorphism, Genetic
Risk Factors
Spine
Angiotensin I
Angiotensins
Peptidyl-Dipeptidase A

Figure

• 

  Fig. 1 Electrophoresis of polymerase chain reaction products of ACE gene. Lane 1 is the insertion/deletion heterozygote (I/D). Lane 2 is the insertion homozygote (I/I). Lane 3 is the deletion homozygote (D/D). ACE, angiotensin I converting enzyme.

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