Korean J Obstet Gynecol.  1999 Jun;42(6):1196-1200.

Expression of Placental Gelatinases in Normal and Preeclamptic Pregnancy

Abstract


OBJECTIVES
The aim of this study was to compare activities of gelatinases and their regulators in normal and preeclamptic placenta tissue.
METHODS
The activities of gelatinase A and B were evaluated by gelatin zymography in eight preeclamptic and 8 normal placentas. The levels of MMP-2, MMP-9, TIMP-1, TIMP-2 and MTI-MMP were measured by Northern blotting and gelatin zymography.
RESULTS
Densitometric measurements of gelatin zymogram revealed decreased activities of 92 kDa proMMP-9 and 72kDa proMMP-2 activity in preeclamptic placentas compared to normal placentas. The activities of 62 kDa active MMP-2, however, were not different between normal and preeclamptic placentas. The level of MMP-2 and MMP-9 mRNA was lower in preeclamptic placenta than in normal placenta. On the other hand, there was no difference in MT1-MMP and TIMP-2 mRNA level between normal and preeclamptic placenta. To the contrary of our expectation, increased level of poMMP-2 and proMMP-9 activities in placenta was associated with labor.
CONCLUSIONS
Decreased activities of gelatinases may contribute to defective trophoblastic invasion of maternal tissues in preeclampsia. The MT1-MMP ssems to be a main determinant of gelatinase activities in human placenta. The mechanism that regulates activities of other MMPs in human placenta needs further investigations.

Keyword

Placenta; Gelatinases; MMP; Preeclampsia
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