Korean J Obstet Gynecol.
1997 Jan;40(1):154-160.
Inhibition of Cell Growth and Suppression of c-myc Gene Expression by Transforming Growth Factor-beta1 in Cervical Carcinoma Cell Lines
Abstract
- Transforming growth factor-beta1(TGF-beta1) is known to be a potent growth inhibitor for many cell types, including most epithelial cells. In skin keratinocytes, TGF-beta1 has been shown to inhibit growth and to rapidly reduce c-myc expression. However, the molecular mechanism of TGF-beta1 action on cell growth of cervical carcinoma has not yet been elucidated. We thus assessed the effect of TGF-beta1 on the growth of cervical carcinoma cell lines. Two cervical squamous carcinoma cell lines were incubated with varying concentration of TGF-beta 1, and growth inhibition was evaluated with tetrazolium-based colorimetric assay. After culturing in concentrations of 0.1~10 ng/ml in both cell lines. Northern blot analysis revealed c-myc mRNA expression was suppressed by 10 ng/ml of TGF-beta 1 following 6-hour of treatment in both cell lines. These results suggest that TGF-beta1 inhibits the growth of cervical carcinoma cells by suppressing c-myc oncogene expression.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- The Mechanisms of Growth Inhibition by Transforming Growth Factor-beta1 in Cervical Carcinoma Cell Lines
- Effect on Cell Growth, c-myc mRNA Expression and Telomerase Activity by Transforming Growth Factor-beta1 in Malignant Lymphoma and Leukemia Cell Line
- The Expression of Vascular Endothelial Growth Factor, Kinase Domain Region, and Transforming Growth Factor-beta 1 in Cervical Neoplasia
- The Expression of TGF-beta1 and TGF-beta Receptor I in Human Lung Cancer
- The Transforming Growth Factor-beta1 Expression in Normal Laryngeal Mucosa, Laryngeal Dysplasia and Laryngeal Carcinoma
