Korean J Nucl Med.
2000 Aug;34(4):353-359.
The Evaluation of Domestic Immunoradiometric Assay Kit for Alpha-fetoprotein
Abstract
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PURPOSE: Although alpha-fetoprotein is one of the most commonly used tumor markers in Korea,
most of the radioimmunoassay kits for alpha-fetoprotein have been imported from foreign countries.
The purpose of this study was to evaluate the performance of a recently developed domestic
immunoradiometric kit for alpha- fetoprotein (Riakey AFP IRMA CTR, Sin-Jin Medics, Seoul, Korea).
MATERALS AND METHODS: We evaluated intra- and inter-assay precision, recovery rate,
parallelism, and sensitivity of serum alpha-fetoprotein measurement using Riakey AFP IRMA
CTR kit. The values of alpha-fetoprotein measured by Riakey AFP IRMA CTR kit were compared
with those measured by two foreign commercial kits (alpha-fetoproteina of Radim and
alpha-feto.riabead of Abbott).
RESULTS
Intra-assay coefficients of variation on three different levels were 5.3% for
18.9 ng/ml, 3.4% for 133 ng/ml and 1.6% for 330 ng/ml. Inter-assay coefficients of variation
were 9.7% for 20.9 ng/ml, 3.2% for 137 ng/ml and 4.1% for 330 ng/ml respectively. Recovery
rate tests on all three different levels showed within 100+/-10%. Parallelism was also good
and the sensitivity was 0.63 ng/ml. There was strong correlation between the measurement of
alpha-fetoprotein by Riakey AFP IRMA CTR and that by two foreign commercial kits(r=0.98).
CONCLUSION
The first Korean domestic immunoradiometric kit for alpha-fetoprotein,
Riakey AFP IRMA CTR, performed well for clinical use.