Korean J Med.  2008 Nov;75(5):553-558.

Effect of the G-CSF schedule for harvesting peripheral blood progenitor cells from healthy donors

Affiliations
  • 1Department of Internal Medicine, The Catholic University of Korea, College of Medicine, Seoul, Korea. cwp@catholic.ac.kr

Abstract

BACKGROUND/AIMS: Peripheral blood progenitor cells (PBSC) have been used instead of bone marrow cells for allogeneic transplantation. Progenitor cells are mobilized using granulocyte colony stimulating factor (G-CSF). Kinetic studies of G-CSF have shown that the CD34+ cells peak on day 5, although the optimal time for harvesting has not been established.
METHODS
We compared two mobilization schedules using G-CSF. In groups I and II, G-CSF 10 microgram/kg/day was administered subcutaneously for 3 or 4 days, respectively, and leukapheresis was performed the next day.
RESULTS
In groups I and II, a respective mean number of 5.84 x 106 and 10.76 x 106 circulating CD34+ cells per kilogram of the recipient's weight was collected by leukapheresis. The yield of the two collections did not differ significantly (p=0.386).
CONCLUSION
This study suggested that the two mobilization schedules are equally effective. To avoid prolonged exposure to G-CSF, the day 4 collection schedule is the preferred mobilization strategy.

Keyword

Peripheral blood stem cells; Mobilization; Granulocyte colony-stimulating factor; Healthy donor

MeSH Terms

Appointments and Schedules
Bone Marrow Cells
Colony-Stimulating Factors
Granulocyte Colony-Stimulating Factor
Granulocytes
Humans
Leukapheresis
Stem Cells
Tissue Donors
Transplantation, Homologous
Colony-Stimulating Factors
Granulocyte Colony-Stimulating Factor
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