Korean J Blood Transfus.  2000 Jun;11(1):27-34.

ABO gene analysis of cis-AB and B subgroup in blood donors

Affiliations
  • 1Blood Transfusion Research Institute, The Republic of Korea National Red Cross, Seoul, Kyungki Red Cross Blood Center, Suwon.
  • 2Department of Clinical Pathology, Samsung Medical Center, Sungkyunkwan University College of Medicine, Seoul, Korea.

Abstract

BACKGROUDS: Based on elucidation of genetic basis of ABO blood group, the genetic mutation of blood subgroup has been investigated. Especially, the discovery of base substitution such as C467T, G803C in cis-AB have made the efforts to determine cis-AB blood group by molecular method. This study was performed to investigate the ABO gene structure and usefulness of genotyping method in blood donors whose blood group are suspected as cis-AB and B subgroup.
METHODS
Genotyping for ABO was performed in peripheral blood DNA samples from eight cis-AB donors and five B subgroup donors at red cross blood center. DNA sequencing was performed on Bint, B3 and two cis-AB samples. RESLUTS: All eight cis-AB donors showed that they have cis-AB allele and C467T substitution. Through DNA sequencing it was confirmed that cis-AB allele was derived from A allele mutation and two B subgroups showed no base sequence difference with B blood group.
CONCLUSIONS
The genotyping method would be useful tool to determine blood group variants in blood donors. And more investigation is required for elucidation of genetic structure and gene expression of ABO blood subgroup.

Keyword

Cis-AB; B subgroup; Genotyping; Blood donor

MeSH Terms

Alleles
Base Sequence
Blood Donors*
DNA
Gene Expression
Genetic Structures
Humans
Red Cross
Sequence Analysis, DNA
Tissue Donors
DNA
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