Korean J Biol Psychiatry.  2005 May;12(1):42-61.

Gene Expression Profiling of SH-SY5Y Human Neuroblastoma Cells Treated with Ginsenoside Rg1 and Rb1

Affiliations
  • 1Seoul National Hospital, Seoul, Korea.
  • 2Department of Neuropsychiatry, College of Medicine & The Mental Health Research Institute, Hanyang University, Seoul, Korea. bhyang@hanyang.ac.kr
  • 3Division of Molecular and Life Science, College of Science, Hanyang University, Ansan, Korea.
  • 4Department of Psychiatry, College of Medicine, Kwandong University, Myongji Hospital, Goyang, Korea.
  • 5KT & G Central Research Institute, Daejeon, Korea. bhyang@hanyang.ac.kr

Abstract


OBJECTIVES
The ginsenoside Rg1 and Rb1, the major components of ginseng saponin, have neurotrophic and neuroprotective effects including promotion of neuronal survival and proliferation, facilitation of learning and memory, and protection from ischemic injury and apoptosis. In this study, to investigate the molecular basis of the effects of ginsenoside on neuron, we analyzed gene expression profiling of SH-SY5Y human neuroblastoma cells treated with ginsenoside Rg1 or Rb1.
METHODS
SH-SY5Y cells were cultured and treated in triplicate with ginsenoside Rg1 or Rb1(80micrometer, 40micrometer, 20micrometer). The proliferation rates of SH-SY5Y cells were determined by MTT assay and microscopic examination. We used a high density cDNA microarray chip that contained 8K human genes to analyze the gene expression profiles in SH-SY5Y cells. We analyzed using the Significance Analysis of Microarray(SAM) method for identifying genes on a microarray with statistically significant changes in expression.
RESULTS
Treatment of SH-SY5Y cells with 80microliter ginsenoside Rg1 or Rb1 for 36h showed maximal proliferation compared with other concentrations or control. The results of the microarray experiment yielded 96 genes were upregulated(> or =3 fold) in Rg1 treated cells and 40 genes were up-regulated(> or =2 fold) in Rb1 treated cells. Treatment with ginsenoside Rg1 for 36h induced the expression of some genes associated with protein biosynthesis, regulation of transcription or translation, cell proliferation and growth, neurogenesis and differentiation, regulation of cell cycle, energy transport and others. Genes associated with neurogenesis and neuronal differentiation such as SCG10 and MLP increased in ginsenoside Rg1 treated cells, but such changes did not occur in Rb1- group.
CONCLUSION
Our data provide novel insights into the gene mechanisms involved in possible role for ginsenoside Rg1 or Rb1 in mediating neuronal proliferation or cell viability, which can elicit distinct patterns of gene expression in neuronal cell line. Ginsenoside Rg1 have more broad and strong effects than ginsenoside Rb1 in gene expression and related cellular physiology. In addition, we suggest that SCG10 gene, which is known to be expressed in neuronal differentiation during development and neuronal regeneration during adulthood, may have a role in enhancement of activity dependent synaptic plasticity or cytoskeletal regulation following treatment of ginsenoside Rg1. Further, ginsenoside Rg1 may have a possible role in regeneration of injured neuron, promotion of memory, and prevention from aging or neuronal degeneration.

Keyword

Ginsenoside Rg1; Ginsenoside Rb1; Microarray; Gene expression; SCG10

MeSH Terms

Aging
Apoptosis
Cell Cycle
Cell Line
Cell Proliferation
Cell Survival
Gene Expression Profiling*
Gene Expression*
Humans*
Learning
Memory
Negotiating
Neuroblastoma*
Neurogenesis
Neurons
Neuroprotective Agents
Oligonucleotide Array Sequence Analysis
Panax
Physiology
Plastics
Protein Biosynthesis
Regeneration
Saponins
Transcriptome
Neuroprotective Agents
Plastics
Saponins
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