Korean J Dermatol.  2008 Jun;46(6):736-741.

Expression of Peroxiredoxin I in the Epidermis of Vitiligo

Affiliations
  • 1Department of Dermatology, Chonnam National University Medical School, Gwangju, Korea.
  • 2Clinical Trial Center, Chonnam National University Hospital, Gwangju, Korea. schul@chonnam.ac.kr

Abstract

BACKGROUND: Although the pathogenesis of vitiligo isn't fully understood, a recent study demonstrates that oxidative stress plays an important role to induce vitiligo. Peroxiredoxin (Prx) is a novel peroxidase family to remove hydrogen peroxide using thioredoxin system, which is consisted of thioredoxin, thioredoxin reductase, and NADPH.
OBJECTIVE
This study aimed to investigate the change of expression of Prx I to elucidate the role of oxidative stress in the pathogenesis of vitiligo.
METHODS
Sample specimens were obtained from the lesional skin of vitiligo patients, and non-depigmented skin was obtained from the perilesional area as control samples. The skin samples were immediately frozen using liquid nitrogen, and then section samples were prepared to perform immunohistochemical staining with antibodies for Prx I. Some of the skin biopsy samples were used for primary culture of keratinocytes. Protein extracts from the expanded keratinocytes were prepared for Western blot analysis of Prx I.
RESULTS
In vitiligo, the ubiquitous expression of Prx I in all layers of epidermis, which was also observed in the normal perilesional skin, was reduced in the depigmented lesion of vitiligo patients. The reduction of Prx I was remarkable from the lesions which were exposed to sunlight. Consistently, Prx I expression from the lesional keratinocytes were noticeably reduced in comparison with that from perilesional keratinocytes.
CONCLUSION
Our results showing that Prx I is impaired in the epidermis of depigmented lesions of vitiligo patients suggest that oxidative stress is an important factor to induce vitiligo.

Keyword

Oxidative stress; Peroxiredoxin I; Vitiligo

MeSH Terms

Antibodies
Biopsy
Blotting, Western
Epidermis
Humans
Hydrogen Peroxide
Keratinocytes
Nitrogen
Oxidative Stress
Peroxidase
Peroxiredoxins
Skin
Sunlight
Thioredoxin-Disulfide Reductase
Thioredoxins
Vitiligo
Antibodies
Hydrogen Peroxide
Nitrogen
Peroxidase
Peroxiredoxins
Thioredoxin-Disulfide Reductase
Thioredoxins
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