Korean J Asthma Allergy Clin Immunol.  2008 Sep;28(3):199-204.

Difference of Dermatophagoides farinae Specific IgE Binding Patterns between Asthmatics and Atopic Dermatitis Patients

Abstract

BACKGROUND: Dermatophagoides farinae (D. farinae) has various isoallergens. However, allergenic differences between bronchial asthmatics (BA) and atopic dermatitis (AD) patients have not yet been fully evaluated.
OBJECTIVE
To investigate the differences in D. farinae specific IgE between BA and AD patients sensitized to D. farinae. METHOD: Five BA and five AD patients sensitized to D. farinae were measured in this study. D. farinae-specific IgE was evaluated by 1-dimensional (1-D) and 2-dimensional (2-D) SDS-PAGE, and IgE immunoblotting. N-terminal sequencing was performed for the identification of the allergens. RESULT: Two-D IgE immunoblot revealed the 7 allergens with 14 kDa at PI 5.5 to 6.5. These allergens were found in 4, BA and 2 AD patients. Another allergen with 25 kDa at pI 7.8 was found only in 4 BA patients. This allergen was regarded as triosephosphate isomerase by N-terminal sequencing.
CONCLUSION
Seven allergens with 14 kDa at pI 5.5 to 6.5 and 1 allergen with 25 kDa at pI 7.8 were identified more frequently in BA than in AD patients. The 25 kDa allergen was regarded as triosephosphate isomerase, and it might be associated with the development of asthma.

MeSH Terms

Allergens
Asthma
Dermatitis, Atopic
Dermatophagoides farinae
Electrophoresis, Polyacrylamide Gel
Humans
Immunoblotting
Immunoglobulin E
Pyroglyphidae
Triose-Phosphate Isomerase
Allergens
Immunoglobulin E
Triose-Phosphate Isomerase