Korean Circ J.  1999 Dec;29(12):1332-1340. 10.4070/kcj.1999.29.12.1332.

Significance of eNOS Gene Polymorphism for the Prediction of Restenosis after Coronary Angioplasty in Patients with Ischemic Heart Disease

• Choi SY
• Chae IH
• Kim HS
• Son DW
• Oh BH
• Lee MM
• Park YB
• Choi YS
• Lee YW

• KMID: 2228827
• DOI: http://doi.org/10.4070/kcj.1999.29.12.1332

Abstract

BACKGROUND: The restenosis after coronary angioplasty is the unresolved problem even if the improvement of interventional skills and pharmacological therapies. Nitric oxide, known as endothelial derived relaxing factor (EDRF), regulates the vascular tone and inhibits the proliferation of vascular smooth muscle cells and platelet adhesions and endothelium-leukocyte interactions. Nitric oxide is produced by endothelial nitric oxide synthase (eNOS). We studied the significance of eNOS gene polymorphism for the prediction of restenosis after coronary angioplasty in Koreans with ischemic heart disease.
METHODS
We analyzed the two eNOS poly-morphisms using PCR (eNOS A/B polymorphism is the VNTR in intron 4 and eNOS T/G polymorphism is a missense mutation in exon 7) in 199 Korean patients who had 257 lesions undergoing percutaneous coronary angioplasty (ballooning=152, stenting=105). The angiography was repeated 6 months later to assess the relation between the rate of restenosis and types of eNOS gene polymorphism.
RESULTS
We found no significant differences of restenosis rate in eNOS A/B and T/G polymorphism in those with balloon angioplasty or with stent (restenosis rate of A/A, A/B, B/B, respectively (n=257): 25% (1/4), 26% (14/53), 31% (62/200) (p=not significant), and T/T, T/G, G/G (n=249): 0% (0/3), 36% (16/44), 29% (58/202)(p=not significant)) Patients with A allele (non BB) or GG phenotype had lower restenosis rate, so we analyzed protective effect of non BB and GG phenotype on restenosis, but there was no significant statistical difference (restenosis rate of non BB and GG, BB and non GG respectively: 20% (15/57), 34% (16/47)(p=not significant)).
CONCLUSION
eNOS A/B and T/G polymorphism is not associated with a significantly elevated risk of restenosis after coronary angioplasty.

Keyword

Endothelial nitric oxide synthase gene polymorphism; Restenosis; Coronary angioplasty

MeSH Terms

Alleles
Angiography
Angioplasty*
Angioplasty, Balloon
Blood Platelets
Exons
Humans
Introns
Muscle, Smooth, Vascular
Mutation, Missense
Myocardial Ischemia*
Nitric Oxide
Nitric Oxide Synthase Type III
Phenotype
Polymerase Chain Reaction
Stents
Nitric Oxide
Nitric Oxide Synthase Type III