Korean J Anat.  2004 Jun;37(3):283-292.

Localization of Motor Neuronal Cell Bodies Innervating the Digastric Muscle of the Rat Using Cholera Toxin B Subunit (CTB)

Affiliations
  • 1Department of Anatomy and Institute for Medical Science, Chonbuk National University Medical school, Jeonju, Korea. han@chonbuk.ac.kr

Abstract

We used cholera toxin B subunit (CTB) as a neural tracer to localize motor neuronal cell bodies innervating the digastric muscle. After CTB injection into the left anterior belly, CTB-labelled motor neuronal cell bodies were found in caudal half of the left and right trigeminal nucleus, the left and right facial nucleus, the accessory facial nucleus and the accessory trigeminal nucleus in pons. The total number of CTB-labelled motor neuronal cell bodies were 1,179+/-119.5 in the left pons and 246+/-61.8 in the right pons after CTB injections into the left anterior belly of digastric muscle. After CTB injection into left posterior belly, CTB-labelled motor neuronal cell bodies were found only in the left ventral part of accessory facial nucleus in caudal pons and the total number of CTB-labelled motor neuronal cell bodies were 270+/-29.3.

Keyword

CTB; Digastric muscle; Neural tracer

MeSH Terms

Animals
Cholera Toxin*
Cholera*
Motor Neurons*
Pons
Rats*
Trigeminal Nuclei
Cholera Toxin
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