J Rhinol.  1999 May;6(1):24-29.

Expression of GABA Transporters in the Rat's Olfactory Bulb during Development

Affiliations
  • 1Department of Otolaryngology-Head and Neck Surgery, Korea. yongjinp@yahoo.com
  • 2Anatomy, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Abstract

Developmental changes in the expression of two GABA (gamma-aminobutyric acid) transporter proteins, GAT-1 and GAT-3, in the olfactory bulb of embryonic and postnatal rats were examined with immunocytochemistry using antisera against GAT-1 and GAT-3. The expression and localization of GAT-1 and GAT-3 showed distinct temporal patterns during olfactory bulb development. GAT-1 immunoreactivity appeared weakly in most likely growing axons of the presumptive glomerular layer from embryonic day 18 and increased during the first postnatal week. In contrast, GAT-3 immunoreactivity, first detected at E16, was found in radial glial cell fascicles and was replaced by what were likely astroglial cells postnatally. At P7, GAT-1 and GAT-3 immunoreactivities reached the adult pattern i.e., GAT-1 immunoreactivity was observed in the labeled punctate structures in all layers of the olfactory bulb except the nerve fiber layer, while GAT-3 immunoreactivity was observed in the astroglial processes of all layers of the olfactory bulb. Our results suggest that GABA transporters, especially GAT-3, play important roles in regulating the GABA levels of developing olfactory bulbs.

Keyword

GABA transporter (GAT-1, GAT-3); Immunocytochemistry; Olfactory bulb; Development; Rat
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