Dement Neurocogn Disord.  2013 Mar;12(1):1-8. 10.12779/dnd.2013.12.1.1.

Preliminary Study for a Multicenter Study of Alzheimer's Disease Cerebrospinal Fluid Biomarkers

• Park SA
• Kim JH
• Kim HJ
• Kim TE
• Kim YJ
• Lee DH
• Park JH
• Chae WS
• Yim SJ
• Seo SW
• Na DL
• Choi SH

• KMID: 2222355
• DOI: http://doi.org/10.12779/dnd.2013.12.1.1

Abstract

BACKGROUND
The usefulness of cerebrospinal fluid (CSF) concentrations of amyloid beta protein 1-42 (Abeta42), phosphorylated tau (pTau) and total tau (tTau) have been increasing in Alzheimer's disease (AD). However, the direct adoption of previously reported standard values is not appropriate due to interlaboratory variability. We started this study to set up an accessible system to measure CSF biomarkers in our country with high reproducibility and validity.
METHODS
Including CSFs from four different institutes the levels of Abeta42, pTau181 and tTau were measured in one lab. The intertest variability and difference in the levels of biomarkers depending on diseases were assessed. Through analysis of receiver operating characteristic cut points and binary logistic regression the cut-off values of Abeta42, pTau and tTau level were obtained, and their validity was evaluated.
RESULTS
The intertest consistency was high in measuring CSF biomarkers. The value of Abeta42 was markedly decreased in AD (n= 17) and other dementia (n= 9) compared to normal control (n= 12). The levels of pTau181 and tTau were high in AD, but not in other dementia and normal control. The threshold values of Abeta42, pTau181 and tTau were 290.3 pg/mL, 54.3 pg/mL, and 320.7 pg/mL in differentiating AD from normal control showing high sensitivity and specificity. Especially, the ratios of pTau181/Abeta42 (> 0.16) and tTau/Abeta42 (> 0.76) showed the prime validity.
CONCLUSIONS
Our data of CSF Abeta42, pTau181, and tTau levels were highly reproducible. PTau181/Abeta42 and tTau/Abeta42 ratios were the greatly helpful in differentiating AD from normal control.

Keyword

Alzheimer's disease; Amyloid beta protein; Biomarker; Enzyme-linked immunosorbent assay; Cerebrospinal fluid; Tau

MeSH Terms

Academies and Institutes
Adoption
Alzheimer Disease
Amyloid beta-Peptides
Biomarkers
Dementia
Enzyme-Linked Immunosorbent Assay
Logistic Models
Pyridines
ROC Curve
Sensitivity and Specificity
Thiazoles
Amyloid beta-Peptides
Pyridines
Thiazoles

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