J Korean Ophthalmol Soc.  2014 Feb;55(2):278-282. 10.3341/jkos.2014.55.2.278.

Evaluation of the Stabilization of Human Umbilical Cord Blood-Derived Mast Cells in Accordance with Ketotifen and Olopatadine Concentration

• Chun JW
• Koh JW

• KMID: 2218423
• DOI: http://doi.org/10.3341/jkos.2014.55.2.278

Abstract

PURPOSE
To evaluate the effect of olopatadine and ketotifen to stabilize mast cells using human umbilical cord blood-derived mast cells (hCBMCs).
METHODS
Using cultured hCBMCs, we divided the cells into the Ketotifen fumarate treatment group, the Olopatadine hydrochloride treatment group, the positive control group, and the negative control group. The histamine release inhibition rate was then observed.
RESULTS
Ketotifen and olopatadine both showed the highest inhibition rate of histamine release at a concentration of 10(-3.5)M (Ketotifen, 48% and Olopatadine, 62%). The histamine release inhibition rate of olopatadine was 28% at a concentration of 10(-5.5)M, but ketotifen demonstrated a low histamine release inhibition rate at the same concentration. Ketotifen and olopatadine showed no histamine release inhibition at concentrations of 10(-2)~10(-2.5)M, and 10(-6)M.
CONCLUSIONS
Ketotifen and olopatadine demonstrated histamine inhibition in the concentration range of 10(-3) to 10(-5)M. Olopatadine showed a slightly stronger response than ketotifen in the inhibition of histamine release.

Keyword

Histamine; Ketotifen; Mast cell; Olopatadine

MeSH Terms

Histamine
Histamine Release
Humans*
Ketotifen*
Mast Cells*
Umbilical Cord*
Olopatadine Hydrochloride
Histamine
Ketotifen