J Korean Ophthalmol Soc.  2011 Jan;52(1):86-92. 10.3341/jkos.2011.52.1.86.

The Study of Characteristics of Acellular Porcine Cornea Using Freezing-Thawing-Centrifugation

Affiliations
  • 1Department of Ophthalmology, Chungang University Yongsan Hospital, Seoul, Korea. jck50ey@kornet.net

Abstract

PURPOSE
To develop a new decellularization technique of porcine cornea using freezing-thawing-centrifugation (FTC) and to examine the characteristics of acellular porcine cornea (APC) for xenograft material.
METHODS
Two-hundred micrometer thickness porcine corneas were decellularized with DNase/RNase, followed by 3 freezing-thawing-centrifugations (FTC, group 1), lyophilized FTC-APC (group 2), and chemical enzyme treated APC (CE-APC, group 3). Histologic evaluation to examine cells and collagen matrix, comparison of transparency, and cultivation to determine the viability of stromal cells was performed in fresh porcine cornea and 3 experimental groups.
RESULTS
Decellularization occurred successfully in all experimental groups. Decellularization was confirmed by H&E staining and cultivation. Transparency of group 1 was similar to the normal porcine cornea but transparency of group 2 and group 3 was decreased. Collagen fibers of CE-APC (group 3) were not as well arrayed as FTC-APC (group 2).
CONCLUSIONS
Acellularity of porcine cornea was successfully achieved by the FTC method with preservation of the cornea stroma. Novel decellularized porcine cornea can be considered as xenogeneic material for corneal transplantation.

Keyword

Centrifuge; Decellularization; Porcine cornea; Xenograft

MeSH Terms

Collagen
Cornea
Corneal Transplantation
Stromal Cells
Transplantation, Heterologous
Collagen
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