J Nutr Health.  2015 Dec;48(6):468-475. 10.4163/jnh.2015.48.6.468.

Oxidative damage biomarker levels according to Mn-SOD and GST gene polymorphisms in preschool children

• Shin YK
• Choi JW
• Oh SY
• Chung J

• KMID: 2209860
• DOI: http://doi.org/10.4163/jnh.2015.48.6.468

Abstract

PURPOSE
Genetic polymorphisms in antioxidant defense and detoxification genes may modulate the levels of oxidative stress biomarkers.
METHODS
A total of 301 healthy preschool-aged children in the Seoul and Kyung-gi areas were recruited. DNA was extracted from blood for genotyping of manganese superoxide dismutase (Mn-SOD) Val16Ala, glutathione S-transferase (GST) P1 Ile105Val, GSTT1 present/null, and GSTM1 present/null polymorphisms by PCR-restriction fragment length polymorphism or multiplex PCR analyses. In addition to a questionnaire survey, the levels of urinary 8- hydroxyl-2-deoxiguanosine (8-OHdG) and plasma malondialdehyde (MDA) were measured by ELISA.
RESULTS
Significantly higher urinary 8-OHdG concentrations were observed in GSTP1 Ile/Val + Val/Val genotype (p = 0.030), and tended to be higher in Mn-SOD Val/Val genotype (p = 0.065). On the other hand, exposure to environmental tobacco smoking (ETS) and interaction between ETS and gene polymorphisms did not significantly influence either urinary 8-OHdG concentrations or serum MDA.
CONCLUSION
Based on our findings, GSTP1 Ile/Val gene polymorphisms might modulate the levels of oxidative stress biomarkers in healthy preschool children.

Keyword

Genetic polymorphism; Mn-superoxide dismutase; glutathione S-transferase; 8-hydroyxyl-2-deoxyguanosine

MeSH Terms

Biomarkers
Child
Child, Preschool*
DNA
Enzyme-Linked Immunosorbent Assay
Genotype
Glutathione Transferase
Gyeonggi-do
Hand
Humans
Malondialdehyde
Multiplex Polymerase Chain Reaction
Oxidative Stress
Plasma
Polymorphism, Genetic
Seoul
Smoking
Superoxide Dismutase*
DNA
Glutathione Transferase
Malondialdehyde
Superoxide Dismutase

Figure

• 

  Fig. 1. The levels of urinary 8-hydroxyl-2-deoxyguanosine (8-OHdG) according to exposure to environmental tobacco smoking (ETS) and gene polymorphisms. [(A) Mn-SOD, (B) GSTP1, (C) GSTT1, and (D) GSTM1] Data were analyzed using GLM models adjusted for age, sex, and nutrient supplement intake.

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