J Korean Soc Plast Reconstr Surg.  1998 Oct;25(7):1219-1225.

Effect of bone wax plus bone morphogenetic protein into rabbit calvarial defects

• No authors listed

Abstract

Bone is a storehouse of biologic factors enabling it to regenerative without scar formation. Recombinant technology has made many of these factors available in significant quality for therapeutic applications. However, a delivery system of recombinant bone regenerating factors is needed. Biodegradable, biocompatable polymers have made it possible for delivering bone regenerative factors, such as bone morphogenetic protein(BMP). The overall goal of this study was to assess an experimental biodegradable polymer with wax-like handling properties that was combined with recombinant simian transforming growth factor beta(rs TGF-beta). The purpose of this study was to validate biodegradability, biocompatability, hemostasis and bone promotion within standard-sized defects. The experimental design included 24 skeletally mature New-Zealand white rabbit divided evenly between two time periods (6 and 12 weeks) and the experimental wound model was two standard-sized circular defects 8 mm in diameter on each side of rabbit calvaria. At necropsy, a rectangular-shape segment of the calvaria was removed that included the parietal bones and defect sites. The segment was radiographed and x-ray films were assessed for radiopacity by computer assisted imaging analysis technique(i.e, radiomorphometry). Following radiography, bone formation within the defects was quantified by a computer imaging technique(hismtorphometry).Radiopacity associated with the experimental bone wax polymers was significantly greater than that of the untreated controls. Quantitative histomorphometry revealed the amount of new bone within the untreated controls was significantly greater than the experimentally treated sites. As a result, bone-wax did not satisfy the condition of ideal carrier of BMP but had some advantage in clinical use. The follow-up study must be produced for improvement of the substitution of bone with BMP in carrier.

MeSH Terms

Biological Factors
Bone Morphogenetic Proteins*
Cicatrix
Follow-Up Studies
Hemostasis
Osteogenesis
Parietal Bone
Polymers
Radiography
Research Design
Skull
Transforming Growth Factors
Wounds and Injuries
X-Ray Film
Biological Factors
Bone Morphogenetic Proteins
Polymers
Transforming Growth Factors