J Korean Soc Transplant.
2007 Jun;21(1):31-37.
Human Umbilical Cord Blood-derived Cells Generate Insulin-producing Cells In Vitro
- Affiliations
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- 1Stem Cell Therapy Center, Soonchunhyang University Hospital, Korea. dhchoi@hosp.sch.ac.kr
- 2Institute for Clinical Molecular Biology Research, Soonchunhyang University College of Medicine, Korea.
- 3Department of Surgery, College of Medicine, Soonchunhyang University, Seoul, Korea. dhchoi@hosp.sch.ac.kr
- 4Department of Hematology-Oncology, College of Medicine, Soonchunhyang University, Seoul, Korea.
- 5Department of Endocrinology, College of Medicine, Soonchunhyang University, Seoul, Korea.
Abstract
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PURPOSE: Here we showed that human umbilical cord blood (hUCB)-derived cells, when cultured under defined conditions, generated insulin-producing cells (IPCs).
METHODS
hUCB mononuclear cells (MNCs) were cultured in serum-free low (5.5 mM glucose) DMEM at a cell density of 3x10(6)/cm2 in the presence of 1% DMSO for 3 days followed by high (25 mM glucose) DMEM supplemented with 10% FBS for 7 additional days. They were plated in plastic six well plates on slide coverslips (22x22 mm2) coated with 0.006% type I collagen.
RESULTS
These IPCs formed clusters similar to islets of Langerhans. We confirmed these clusters were positive for insulin and C-peptide by immunohistochemistry.
CONCLUSION
Our data demonstrated that in vitro hUCB-derived cells generated IPCs, which can be a potential source for the treatment of diabetes via a stem cell therapy approach.