J Korean Soc Transplant.  2004 Dec;18(2):134-139.

Analysis of Pretransplant ELISA-Panel Reactive Antibody in Kidney Transplant Patients

• Oh EJ
• Park YJ
• Lee KY
• Choi BS
• Yang CW
• Kim YS
• Bang BK
• Koh YB
• Kang CS

Abstract

BACKGROUND
HLA antibodies have been shown to be associated with graft loss of organ transplants in prior studies. This study was designed to analyze the results of ELISA- panel reactive antibody (ELISA-PRA) in kidney transplant patients and the impact of this test on the clinical outcome.
METHODS
We have investigated ELISA-PRA results from 110 living donor renal transplant patients from Nov. 2001 to Apr. 2004.
RESULTS
ELISA-PRA positivity was found in 22 (20%) patients and was higher in the female patients than male (P<0.05). Pretransplant transfusion, pregnancy or transplantation history was not significantly correlated with ELISA- PRA result. ELISA-PRA (+)patients had more rejection episodes of 41% (n=9) (P=0.0005) and graft failures of 18% (n=4) (P=0.028) than ELISA-PRA (-), which had 8% (n=7) and 3% (n=3), respectively. Patients group with a result of ELISA-PRA/flowcytometric crossmatch (FCXM) (+/ -) or (+ / +) had worse clinical outcome than ELISA- PRA/FCXM ( -/- ). ELISA-PRA/FCXM (+ /+ ) correlated with higher incidence of allograft rejection than ELIS- PRA/FCXM (+ /- ) or ( -/+ ).
CONCLUSION
These results suggest that in conjunction with FCXM results, pretransplant ELISA-PRA test is useful predictor of clinical outcome in renal transplant recipients.

Keyword

Kidney; Transplantation; Panel reactive antibody (PRA); ELISA; Rejection; Crossmatch

MeSH Terms

Allografts
Antibodies
Enzyme-Linked Immunosorbent Assay
Female
Humans
Incidence
Kidney*
Living Donors
Male
Pregnancy
Transplantation
Transplants
Antibodies