J Korean Assoc Oral Maxillofac Surg.  2007 Apr;33(2):81-93.

An Experimental Study for Establishment of Orthotopic Salivary Tumor Models in Mice

Affiliations
  • 1Department of Oral and Maxillofacial Surgery, College of Dentistry, Kangnung National University, Korea. ywpark@kangnung.ac.kr

Abstract

PURPOSE: Adenoid cystic carcinoma (ACC) is a relatively rare tumor that arises in glandular tissues of the head and neck region and sometimes has a protracted clinical course with perineural invasion and delayed onset of distant lung metastasis. Treatment failure of salivary ACC is most often associated with perineural and hematogenous tumor spread. However, very little has been known about the cellular and molecular mechanisms of perineural invasion and hematogenous distant metastasis of parotid ACC. This study was designed to develop an orthotopic tumor model of parotid adenoid cystic carcinoma in athymic nude mice. EXPERIMENTAL DESIGN: A melanoma cell line was injected into the parotid gland of athymic mice to determine whether such implantation was technically feasible. A parotid ACC cell line was then injected into the parotid gland or the subcutaneous tissue of athymic mice at various concentrations of tumor cells, and the mice were thereafter followed for development of tumor nodule. The tumors were examined histopathologically for perineural invasion or regional or distant lung metastasis. We used an oral squmous cell carcinoma cell line as control.
RESULTS
Implantation of tumor(melanoma) cell suspension into the parotid gland of nude mice was technically feasible and resulted in the formation of parotid tumors. A parotid ACC cell line, ACC3 showed no significantly higher tumorigenicity, but showed significantly higher lung metastatic potential in the parotid gland than in the subcutis. In contrast, mucosal squmous cell carcinoma cell line doesn't show significantly higher lung metastatic potential in the parotid gland than in the subcutis. The ACC tumor established in the parotid gland seemed to demonstrate perineural invasion of facial nerve, needs further study.
CONCLUSION
An orthotopic tumor model of salivary ACC in athymic nude mice was successfully developed that closely recapitulates the clinical situations of human salivary ACC. This model should facilitate the understanding of the cellular and molecular mechanisms of tumorigenisis and metastasis of salivary ACC and aid in the development of targeted molecular therapies of salivary ACC.

Keyword

Adenoid cystic carcinoma; Orthotopic tumor model; Lung metastasis; Cellular and molecular mechanisms

MeSH Terms

Animals
Carcinoma, Adenoid Cystic
Cell Line
Facial Nerve
Head
Humans
Lung
Melanoma
Mice*
Mice, Nude
Molecular Targeted Therapy
Neck
Neoplasm Metastasis
Parotid Gland
Research Design
Subcutaneous Tissue
Treatment Failure
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