J Korean Orthop Assoc.  2010 Feb;45(1):65-72. 10.4055/jkoa.2010.45.1.65.

Gene Expression in the Capsule Tissue of the Frozen Shoulder

• Kim JM
• Kim YS
• Ji JH
• Lee JY
• Yoo HH
• Lee YK

• KMID: 2185596
• DOI: http://doi.org/10.4055/jkoa.2010.45.1.65

Abstract

PURPOSE
To investigate the overall expression of extracellular matrix (ECM) and adhesion molecule genes using a gene array technique in the joint capsule of a frozen shoulder.
MATERIALS AND METHODS
Tissues from 20 human shoulder joint capsules were harvested intraoperatively from patients (15 primary frozen shoulders, 5 controls) in our hospital. The RNA was isolated from the capsule tissue and the gene expression of ECM and adhesion molecules was analyzed using an oligo-array technique.
RESULTS
The expression of several genes of the ECM and cell adhesion molecules was significantly higher in the capsule tissue from patients with a frozen shoulder than the controls. The gene expression of the collagen V alpha1/alpha3, VI alpha2/alpha3, VIII alpha1/alpha2, XV alpha1, XVIII alpha1 and ECM proteins including CD 44, connective tissue growth factor (CTGF), matrix metalloproteinase (MMP)-9/14, osteonectin, veriscan, hyaluronan synthase (HAS)-1, extra-cellular matrix (ECM)-1, secreted phosphoprotein (SSP)-1, tenascin C (TNC), thrombospondin 2/4 was two times higher in the frozen shoulder than the control. Several cell adhesion molecules genes including catenin alpha1, seletin p, integrin alpha3, beta2, beta4, beta5 and laminin alpha4, alpha5 were also two times higher in the in the patients with a frozen shoulder than the control.
CONCLUSION
The gene expression of several ECM proteins and adhesion molecules is significantly higher in patients with frozen shoulder.

Keyword

frozen shoulder; oligo-array; extracellular matrix; adhesion molecule

MeSH Terms

Bursitis
Capsules
Cell Adhesion Molecules
Collagen
Connective Tissue Growth Factor
Extracellular Matrix
Gene Expression
Genes, vif
Glucuronosyltransferase
Humans
Hyaluronic Acid
Integrin alpha3
Joint Capsule
Laminin
Osteonectin
Proteins
RNA
Shoulder
Shoulder Joint
Tenascin
Thrombospondins
Capsules
Cell Adhesion Molecules
Collagen
Connective Tissue Growth Factor
Glucuronosyltransferase
Hyaluronic Acid
Integrin alpha3
Laminin
Osteonectin
Proteins
RNA
Tenascin
Thrombospondins

Figure

• 

  Figure 1 Representative examples of microarray blots of human ECM and adhesion molecules from capsule tissue of the frozen shoulder patient and control. (A) Each spot on the microarray membrane represents the expression of an individual gene. For example, the red arrow indicate the MMP-9 gene on the membrane, which is expressed as dark black dot on the frozen shoulder compared with gray dot on the control shoulder. (B) The map of 128 genes on the membrane of GEArray® Series for Human Extracellular Matrix & Adhesion Molecules (Bioscience Corporation, Fredrick, MD). β-2-microglobulin (B2M) and β-actin (ACTB) gene are considered as a control genes, which should have the same expression on both membranes.

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