J Dent Rehabil Appl Sci.  2015 Sep;31(3):212-220. 10.14368/jdras.2015.31.3.212.

Antifungal effect of electrolyzed hydrogen water on Candida albicans biofilm

Affiliations
  • 1Department of Oral Microbiology and Immunology, College of Dentistry, Dankook University, Cheonan, Republic of Korea. micro94@gmail.com
  • 2Dental Research Institute, Dankook University, Cheonan, Republic of Korea.

Abstract

PURPOSE
Candida albicans can cause mucosal disease in many vulnerable patients. Also they are associated with denture-related stomatitis. Electrolyzed water is generated by electric current passed via water using various metal electrodes and has antimicrobial activity. The aim of this study was to investigate antifungal activity of electrolyzed water on C. albicans biofilm.
MATERIALS AND METHODS
C. albicans was cultured by sabouraud dextrose broth and F-12 nutrient medium in aerobic and 5% CO2 condition to form blastoconidia (yeast) and hyphae type, respectively. For formation of C. albicans biofilm, C. albicans was cultivated on rough surface 6-well plate by using F-12 nutrient medium in CO2 incubator for 48 hr. After electrolyzing tap water using various metal electrodes, the blastoconidia and hyphal type of C. albicans were treated with electrolyzed water. C. albicans formed blastoconidia and hyphae type when they were cultured by sabouraud dextrose broth and F-12 nutrient medium, respectively.
RESULTS
The electrolyzed water using palladium electrode (EWP) exhibited antifungal effect on blastoconidia of C. albicans. Also, the EWP significantly has antifungal activity against C. albicans biofilm and hyphae. In the electrolyzed water using various metal electrodes, only the EWP have antifungal activity.
CONCLUSION
The EWP may use a gargle solution and a soaking solution for prevention of oral candidiasis and denture-related stomatitis due to antifungal activity. (

Keyword

electrolyzed water; antifungal activity; Candida albicans; Candidal biofilm
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