J Korean Diabetes Assoc.  2006 May;30(3):161-169. 10.4093/jkda.2006.30.3.161.

Effects of Caloric Restriction on the Expression of PGC-1 and PPARs mRNA in Liver of Otsuka Long-Evans Tokushima Fatty Rats

• Kim SY
• Kim JH
• Bae HY
• Lee BR

• KMID: 2177607
• DOI: http://doi.org/10.4093/jkda.2006.30.3.161

Abstract

BACKGROUND: Gluconeogenesis is strongly stimulated during fasting and is aberrantly activated in diabetes mellitus. PPARgamma-coactivator 1 (PGC-1) and Peroxisome proliferator -activated receptors (PPARs) costimulate the expression of key enzymes of gluconeogenetic pathway. This study was performed to evaluate the response to dietary caloric restriction (CR) on the PPARs and PGC-1 expression in liver of diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats.
METHODS
Diabetic OLETF rats (male, 24 weeks) and Long-Evans Tokushima Otsuka (LETO) rats (male, 24 weeks) were used in this study. Liver PPARs and PGC-1 mRNA, and blood glucose levels were investigated at 1, 2, and 3 weeks after the beginning of 30% CR. PPARs and PGC-1 mRNA were determined by RT-PCR and blood glucose levels were measured by spectrophotometric assay.
RESULTS
The liver PGC-1 mRNA expressions were increased to 19% in non-diabetic LETO rats but significant change was not observed in diabetic OLETF rats by 30% CR. The liver PPARgamma mRNA expressions were not changed in non-diabetic LETO rats but increased to 23% in diabetic OLETF rats by 30% CR. The difference of PPARalpha and PPARbeta mRNA expressions in liver of OLETF and LETO rats were not observed.
CONCLUSION
The liver PPARgamma and PGC-1 expression response to CR are altered in OLETF rats compared to in LETO rats. These findings suggested that PPARgamma and PGC-1 expression control system altered in diabetic OLETF rat liver and altered PPARgamma and PCG-1 expression may some roles on the aberrantly activated gluconeogenesis in diabetes mellitus.

Keyword

Caloric restriction; OLETF rats; PGC-1; PPARgamma

MeSH Terms

Animals
Blood Glucose
Caloric Restriction*
Diabetes Mellitus
Fasting
Gluconeogenesis
Liver*
Peroxisome Proliferator-Activated Receptors*
Peroxisomes
PPAR alpha
PPAR gamma
PPAR-beta
Rats*
Rats, Inbred OLETF
RNA, Messenger*
Blood Glucose
PPAR alpha
PPAR gamma
PPAR-beta
Peroxisome Proliferator-Activated Receptors
RNA, Messenger

Figure

• 

  Fig. 1 Effects of caloric restriction on the body weight of LETO and OLETF rats. Data are means ± SEM. *: P < 0.01.

• 

  Fig. 2 mRNA levels of PGC-1 in whole liver homogenates from Caloric-restricted LETO and OLETF rats (FD). Data are the ratio of the target mRNA to PGC-1 mRNA. The top portion of each figure is a gel representative of n = 3/group. Data are means ± SEM. *: P < 0.01.

• 

  Fig. 3 mRNA levels of PPARα in whole liver homogenates from Caloric-restricted LETO and OLETF rats (FD). Data are the ratio of the target mRNA to PPARα mRNA. The top portion of each figure is a gel representative of n = 3/group. Data are means ± SEM. *: P < 0.05.

• 

  Fig. 4 mRNA levels of PPARβ in whole liver homogenates from Caloric-restricted LETO and OLETF rats (FD). Data are the ratio of the target mRNA to PPARβ mRNA. The top portion of each figure is a gel representative of n = 3/group. Data are means ± SEM. *: P < 0.05.

• 

  Fig. 5 mRNA levels of PPARγ in whole liver homogenates from Caloric-restricted LETO and OLETF rats (FD). Data are the ratio of the target mRNA to PPARγ mRNA. The top portion of each figure is a gel representative of n = 3/group. Data are means ± SEM. *: P < 0.05.

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