J Korean Cancer Assoc.  2000 Jun;32(3):605-611.

Construction of DNA Painting Probe for Transldegrees Cation Region of a Marker Chromosome by Chromosome Microdissection

Affiliations
  • 1Department of Anatomy and Institute for Medical Genetics, School of Medicine, Keimyung University, Daegu, Korea.

Abstract

PURPOSE: Chromosome microdissection has been recommended as a technology to overcome the limited problems of conventional cytogenetic analysis and is a direct approach to isolate DNA from specific interesting region of chromosome. KUMA-1 cell line has a specific reserved chromosome abnormality during prdegrees Cess from primary cancer culture to continuous cell line development, der(2)t(2;?)(qter;?). So molecular analysis for transldegrees Cation region of der(2) may be helpful to understand pathogenesis of this primary cancer. The aim of this study was to develop painting probe for the transldegrees Cation region for molecular study in future about transldegrees Cation region of der(2) of KUMA-1 cell line.
MATERIALS AND METHODS
KUMA-1 cell line was derived from a squamous cell carcinoma of urinary bladder. The transldegrees Cation breakpoint region of der(2) appeared in KUMA-1 cell line was microdissected and dissected chromosome segments were amplified by PCR reaction. Fluorescent in situ hybridization was conducted on KUMA-1 metaphase cells with the probe generated from PCR product to confirm the construction of painting probe containing the transldegrees Cation breakpoint of der(2).
RESULTS
Painting probe was hybridized to the metaphase chromosome of KUMA-1 cell line and two fluorescent signals were mapped to the transldegrees Cation forming chromosomal region of der(2).
CONCLUSION
It was possible to construct the painting probe for the transldegrees Cation region of der (2) by chromosome microdissection.

Keyword

Chromosome microdissection; Marker chromosome; Painting probe; Transldegrees Cation

MeSH Terms

Carcinoma, Squamous Cell
Cell Line
Chromosome Aberrations
Cytogenetic Analysis
DNA*
In Situ Hybridization, Fluorescence
Metaphase
Microdissection*
Paint*
Paintings*
Polymerase Chain Reaction
Urinary Bladder
DNA
Full Text Links
  • JKCA
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr