Int J Oral Biol.  2014 Dec;39(4):201-206. 10.11620/IJOB.2014.39.4.201.

Antimicrobial Activity of Mulberry Leaf against Mutans Streptococci and Periodontopathogens

• Park SN
• Lim YK
• Cho E
• Jo E
• Park PS
• Kook JK

• KMID: 2170788
• DOI: http://doi.org/10.11620/IJOB.2014.39.4.201

Abstract

This study investigated the antimicrobial activity of methanol extract of mulberry leaf against 16 strains of mutans streptococci and four species of periodontopathogens: Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans. The antimicrobial activities of the crude extracts or silica gel chromatography fractions of methanol-extracted mulberry leaf were evaluated by determining minimal inhibitory concentrations using an established microdilution method. The cytotoxicity of the extracts of mulberry leaf on KB cells was tested by the methyl thiazolyl tetrazolium assay. Chromatography fraction 12 displayed the most potent antimicrobial activity against all 16 strains of mutans streptococci, P. gingivalis, and P. intermedia. No KB cell cytotoxicity was evident up to 128 microg/ml of fraction 12. The methanol extract had no antimicrobial activity against F. nucleatum and A. actinomycetemcomitans. These results suggest chromatography fraction 12 methanol extract of mulberry leaf could be useful in the development of oral hygiene products, such as dentifrice and oral hygiene solution, for the prevention of dental caries.

Keyword

antimicrobial effect; mulberry leaf; mutans streptococci; periodontopathogens

MeSH Terms

Aggregatibacter actinomycetemcomitans
Chromatography
Complex Mixtures
Dental Caries
Dentifrices
Fusobacterium nucleatum
Humans
KB Cells
Methanol
Morus*
Oral Hygiene
Porphyromonas gingivalis
Prevotella intermedia
Silica Gel
Complex Mixtures
Dentifrices
Methanol
Silica Gel