Biomol Ther.  2014 May;22(3):246-253.

Neuroprotective Effect of Steamed and Fermented Codonopsis lanceolata

• Weon JB
• Yun BR
• Lee J
• Eom MR
• Ko HJ
• Lee HY
• Park DS
• Chung HC
• Chung JY
• Ma CJ

Abstract

Codonopsis lanceolata has been used as an herbal medicine for several lung infl ammatory diseases, such as asthma, tonsillitis, and pharyngitis. Previously, we showed the neuroprotective effect of steamed and fermented C. lanceolata (SFC) in vitro and in vivo. In the current study, the treatment of HT22 cells with SFC decreased glutamate-induced cell death, suggesting that SFC protected HT22 cells from glutamate-induced cytotoxicity. Based on these, we sought to elucidate the mechanisms of the neuroprotective effect of SFC by measuring the oxidative stress parameters and the expression of Bax and caspase-3 in HT22 cells. SFC reduced contents of ROS, Ca2+ and NO. Moreover, SFC restored contents of glutathione and glutathione reductase as well as inhibited Bax and caspase-3 activity in HT22 cells. These results indicate that steamed and fermented C. lanceolata (SFC) extract protected HT22 cells by anti-oxidative effect and inhibition of the expression of Bax and caspase-3.

Keyword

Codonopsis lanceolata; Steam and fermentation; Neuroprotective activity; Antioxidative activity; Bax; Caspase-3

MeSH Terms

Asthma
Caspase 3
Cell Death
Codonopsis*
Glutathione
Glutathione Reductase
Herbal Medicine
Lung
Neuroprotective Agents*
Oxidative Stress
Palatine Tonsil
Pharyngitis
Steam*
Tonsillitis
Caspase 3
Glutathione
Glutathione Reductase
Neuroprotective Agents
Steam