J Bacteriol Virol.  2016 Mar;46(1):22-26. 10.4167/jbv.2016.46.1.22.

Application of Diagnostic Microarray Technique in Subtyping and Pathotyping of Avian Influenza Viruses Isolated in Mongolia

Affiliations
  • 1Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul, Korea. songcs@konkuk.ac.kr
  • 2Bio-Core Co. Ltd., Seoul, Korea.
  • 3Department of Molecular and Life Science, Hanyang University, Ansan, Gyeonggi-do, Korea.
  • 4Department of Bio-Nanotechnology, Hanyang University, Gyeonggi-do, Korea.

Abstract

Asian-lineage H5 highly pathogenic avian influenza (HPAI) viruses have caused continuous outbreaks in poultry and wild birds. Development of rapid and accurate diagnostic methods is needed for preventing further spread of the virus and reducing the time required for eradication of the virus. We developed a low-density microarray for the rapid detection and identification of avian influenza virus subtypes H5, H7, and H9 and their pathotypes in a previous study. In the present study, we evaluated previously developed diagnostic microarray using avian influenza viruses isolated in Mongolia, including H5 HPAI viruses. All H5 HPAI viruses isolated in Mongolia were shown as H5-specific and highly pathogenic pattern in the microarray. H2, H3 and H12 viruses isolated in Mongolia used in this study did not show any H5, H7 and H9 patterns. These results indicated that this diagnostic microarray has enormous potential for the rapid subtyping and pathotyping of influenza viruses, including viruses isolated in Mongolia.

Keyword

Influenza virus; HPAI; Microarray; Mongolia; Rapid diagnosis
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