J Bacteriol Virol.  2012 Dec;42(4):353-356. 10.4167/jbv.2012.42.4.353.

Detection of Neutralizing Antibody Against Japanese Encephalitis Virus in Wild Boars of Korea

Affiliations
  • 1Animal, Plant and Fishery Quarantine Inspection Agency, MIFAFF, Anyang, Korea. yangdk@korea.kr
  • 2College of Veterinary Medicine, JeonBuk National University, JeonJu, Korea.

Abstract

Several species of animals, including horses and pigs, can be infected with Japanese encephalitis virus (JEV). Wild boars (Sus scrofa) are also considered to be an effective amplifying host for JEV in wild environments. In this study, 288 blood samples were collected from wild boars in eight Korean provinces, and antibodies against JEV were detected using a virus neutralizing assay. The results showed that 66.0% (190/288) of wild boars in Korea had neutralizing antibodies against JEV. We found no significant differences in the seroprevalence of JEV among provinces (p > 0.05). The results indicate that wild boars in Korea have been exposed to JEV, suggesting that these boars may play an important role in amplifying and carrying JEV to other regions of Korea. The result of this study may be helpful for planning preventive measures.

Keyword

Japanese encephalitis virus; Virus neutralization; Wild boar

MeSH Terms

Animals
Antibodies
Antibodies, Neutralizing
Asian Continental Ancestry Group
Encephalitis Virus, Japanese
Encephalitis, Japanese
Horses
Humans
Korea
Lifting
Seroepidemiologic Studies
Sus scrofa
Swine
Viruses
Antibodies
Antibodies, Neutralizing

Figure

  • Figure 1 Frequency of distribution of VN titer in 288 serum samples collected from wild boars in Korea.


Reference

1. Campbell GL, Hills SL, Fischer M, Jacobson JA, Hoke CH, Hombach JM, et al. Estimated global incidence of Japanese encephalitis: a systematic review. Bull World Health Organ. 2011. 89:766–774.
2. Fischer M, Hills S, Staples E, Johnson B, Yaich M, Solomon T. Scheld WM, Hammer SM, Hughes JM, editors. Japanese encephalitis prevention and control: advances, challenges, and new initiatives. Emerging infections 8. 2008. Washington: ASM Press;93–124.
Article
3. Duong V, Sorn S, Holl D, Rani M, Deubel V, Buchy P. Evidence of Japanese encephalitis virus infections in swine populations in 8 provinces of Cambodia: implications for national Japanese encephalitis vaccination policy. Acta Trop. 2011. 120:146–150.
Article
4. Nitatpattana N, Le Flohic G, Thongchai P, Nakgoi K, Palaboodeewat S, Khin M, et al. Elevated Japanese encephalitis virus activity monitored by domestic sentinel piglets in Thailand. Vector Borne Zoonotic Dis. 2011. 11:391–394.
Article
5. Yang DK, Oh YI, Kim HR, Lee YJ, Moon OK, Yoon H, et al. Serosurveillance for Japanese encephalitis virus in wild birds captured in Korea. J Vet Sci. 2011. 12:373–377.
Article
6. Cao QS, Li XM, Zhu QY, Wang DD, Chen HC, Qian P. Isolation and molecular characterization of genotype 1 Japanese encephalitis virus, SX09S-01, from pigs in China. Virol J. 2011. 8:472.
Article
7. Gulati BR, Singha H, Singh BK, Virmani N, Kumar S, Singh RK. Isolation and genetic characterization of Japanese encephalitis virus from equines in India. J Vet Sci. 2012. 13:111–118.
Article
8. Seo SW, Sunwoo SY, Hyun BH, Lyoo YS. Detection of antibodies against classical swine fever virus in fecal samples from wild boar. Vet Microbiol. 2012. (in press).
Article
9. Sugiyama I, Shimizu E, Nogami S, Suzuki K, Miura Y, Sentsui H. Serological survey of arthropod-borne viruses among wild boars in Japan. J Vet Med Sci. 2009. 71:1059–1061.
Article
10. Ting SH, See E, Tan HC, Lee MA, Ooi EE. Development of a simplified assay for the detection of neutralizing antibodies to Japanese encephalitis virus. J Virol Methods. 2001. 93:43–47.
Article
11. Watanabe K, Hirokawa C, Kon M, Tamura T, Nishikawa M. Estimation of focus reduction neutralization test for measurement of neutralizing antibody titer against Japanese encephalitis virus. Jpn J Infect Dis. 2008. 61:424–425.
12. Yang DK, Kim BH, Kweon CH, Kwon JH, Lim SI, Han HR. Biophysical characterization of Japanese encephalitis virus (KV1899) isolated from pigs in Korea. J Vet Sci. 2004. 5:125–130.
Article
13. Boadella M, Díez-Delgado I, Gutiérrez-Guzmán AV, Höfle U, Gortázar C. Do wild ungulates allow improved monitoring of flavivirus circulation in Spain? Vector Borne Zoonotic Dis. 2012. 12:490–495.
Article
14. Hirota J, Nishi H, Matsuda H, Tsunemitsu H, Shimiz S. Cross-reactivity of Japanese encephalitis virus-vaccinated horse sera in serodiagnosis of West Nile virus. J Vet Med Sci. 2010. 72:369–372.
Article
15. Mackenzie JS, Gubler DJ, Petersen LR. Emerging flaviviruses: the spread and resurgence of Japanese encephalitis, West Nile and dengue viruses. Nat Med. 2004. 10:S98–S109.
Article
16. Chen WR, Tesh RB, Rico-Hesse R. Genetic variation of Japanese encephalitis virus in nature. J Gen Virol. 1990. 71:2915–2922.
Article
17. Morita K. Molecular epidemiology of Japanese encephalitis in East Asia. Vaccine. 2009. 27:7131–7132.
Article
18. Yun SM, Cho JE, Ju YR, Kim SY, Ryou J, Han MG, et al. Molecular epidemiology of Japanese encephalitis virus circulating in South Korea, 1983-2005. Virol J. 2010. 7:127.
Article
19. Nidaira M, Taira K, Itokazu K, Kudaka J, Nakamura M, Ohno A, et al. Survey of the antibody against japanese encephalitis virus in Ryukyu wild boars (Sus scrofa riukiuanus) in Okinawa, Japan. Jpn J Infect Dis. 2007. 60:309–311.
20. Ohno Y, Sato H, Suzuki K, Yokoyama M, Uni S, Shibasaki T, et al. Detection of antibodies against Japanese encephalitis virus in raccoons, raccoon dogs and wild boars in Japan. J Vet Med Sci. 2009. 71:1035–1039.
Article
21. Sugiura T, Shimada K. Seroepizootiological survey of Japanese encephalitis virus and Getah virus in regional horse race tracks from 1991 to 1997 in Japan. J Vet Med Sci. 1999. 61:877–881.
Article
22. Lim SI, Kweon CH, Tark DS, Kim SH, Yang DK. Serosurvey on Aino, Akabane, Chuzan, bovine ephemeral fever and Japanese encephalitis virus of cattle and swine in Korea. J Vet Sci. 2007. 8:45–49.
Article
Full Text Links
  • JBV
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr