J Bacteriol Virol.  2010 Dec;40(4):171-177. 10.4167/jbv.2010.40.4.171.

Immunoregulation of Murine Immunocytes to Bifidobacteria Strain Isolated from Feces of Healthy Korean Children: IL-10 Release and Proportional Change of CD4+CD25+ Cells

Affiliations
  • 1Immunology and Pathogenic Biology, College of Basic Medicine, Yanbian University, Yanji, China.
  • 2Department of Microbiology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University, Wonju, Korea. kim6@yonsei.ac.kr
  • 3Division of Biological Science and Technology, College of Science and Technology, Institute of Bioproduct, Yonsei University, Wonju, Korea.
  • 4Department of Microbiology, Kwandong University of College of Medicine, Gangneung, Korea.

Abstract

Bifidobacteria is one of the prototypes of probiotics bacteria, normally inhabitating the intestinal tract of humans. To search for a potent immunoregulatory Bifidobacteria strain, we screened the Bifidobacteria strains isolated from the feces of healthy Korean children. The mRNA or protein expression of an anti-inflammatory cytokine, IL-10, from mouse macrophages stimulated with live Bifidobacteria was examined. Of tested strains, Bifidobacteria A28 induced the highest IL-10 gene expression of murine macrophages. To probe immunoregulatory activity of the selected strain on the mice, we evaluated the proportional changes of CD4+CD25+ surface marker in the murine splenocytes. Flow cytometric analysis showed that the overall percentages of CD4+CD25+ cells in A28-treated splenocytes were higher than those of untreated splenocytes. In parallel, IL-10 release from A28-treated mouse peritoneal macrophages and splenocytes was significantly higher than that of untreated control cells. Collectively, the Bifidobacteria A28 strain isolated from the feces of healthy Korean children augments the mRNA or protein expression of IL-10 release from mouse peritoneal macrophages as well as the proportion of CD4+CD25+ cells of naive splenocytes. These provide in vitro scientific clues that Bifidobacteria A28 might be usable for anti-inflammatory disease such as inflammatory bowel disease (IBD).

Keyword

Bifidobacteria; IL-10; Macrophage; CD4+CD25+ cell; Splenocyte

MeSH Terms

Animals
Bacteria
Child
Feces
Gene Expression
Humans
Inflammatory Bowel Diseases
Interleukin-10
Macrophages
Macrophages, Peritoneal
Mice
Probiotics
RNA, Messenger
Sprains and Strains
Interleukin-10
RNA, Messenger

Figure

  • Figure 1. Expression of IL-10 mRNA in RAW 264.7 cells treated with various Bifidus strains. Lane 1, control; Lane 2, LPS 100 ng/ml; Lane 3, B. infantis; Lane 4, B. catenulactum; Lane 5, A1; Lane 6, A2; Lane 7, A5; Lane 8, A13; Lane 9, A14; Lane 10, A16; Lane 11, A28; Lane 12, A34; Lane 13, B17; Lane 14, B2730; Lane 15, YM112

  • Figure 2. Expression of IL-10 mRNA in mouse peritoneal macrophage treated with various Bifidus strains. Lane 1, control; Lane 2, LPS 100 ng/ml; Lane 3, A1; Lane 4, A2; Lane 5, A5; Lane 6, A13; Lane 7, A14; Lane 8, A16; Lane 9, A28; Lane 10, A34; Lane 11, B17; Lane 12, B2730; Lane 13, YM112; Lane 14, B. infantis; Lane 15, B. catenulactum

  • Figure 3. Production of IL-10 by mouse peritoneal macrophages (A) and splenocytes (B) treated with Bifidus strain A28.

  • Figure 4. Proportional increment of CD4+CD25+ T cell in mouse splenocytes treated with Bifidus strain A28. A: CD4+CD25+ expression in A28-untretaed mouse splenocytes; B: CD4+CD25+ expression in A28 (105 cells/ml)-treated mouse splenocytes; C: CD4+CD25+ expression in A28 (106 cells/ml)-treated mouse splenocytes; D: CD4+CD25+ expression in A28 (107 cells/ml)-treated mouse splenocytes; E: Comparative CD4+CD25+ expression in different numbers of A28-treated mouse splenocytes. Data were expressed as Mean ± standard error and analyzed with one-way ANOVA (∗p < 0.05).


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