J Rhinol.  2016 May;23(1):17-23. 10.18787/jr.2016.23.1.17.

Effect of High-Insulin on MUC4, MUC5AC, and MUC5B Expression in Airway Epithelial Cells

Affiliations
  • 1Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, Korea. ydkim@med.yu.ac.kr
  • 2Department of Internal Medicine, College of Medicine, Yeungnam University, Daegu, Korea.
  • 3Regional Center for Respiratory Diseases, Yeungnam University Medical Center, Daegu, Korea.

Abstract

BACKGROUND AND OBJECTIVES
Insulin is a peptide hormone that regulates the metabolism of carbohydrates and fats by promoting the absorption of glucose from the blood to skeletal muscles. Insulin has been reported to be closely related to cardiovascular, respiratory, and endocrine disease. However, the effect of insulin on production of major mucins in human airway epithelial cells has not been reported. Therefore, this study investigated the relationship between high levels of insulin and mucin in human airway epithelial cells.
MATERIALS AND METHODS
This study analyzed the effect of high level of insulin on MUC4, MUC5AC, and MUC5B expression using reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay in human airway epithelial cells.
RESULTS
In human NCI-H292 airway epithelial cells, high level of insulin significant increased MUC4, MUC5AC, and MUC5B mRNA expression and glycoprotein production. In the primary cultures of normal nasal epithelial cells, high level of insulin also increased MUC4, MUC5AC, and MUC5B expression.
CONCLUSION
These results suggest that insulin plays a role in control of mucus hypersecretion in human airway epithelial cells.

Keyword

Insulin; MUC4; MUC5AC; MUC5B; Human airway epithelial cell
Full Text Links
  • JR
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2020 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr