Allergy Asthma Respir Dis.  2016 May;4(3):199-204. 10.4168/aard.2016.4.3.199.

Comparison of IgE-binding components between 2 house dust mites in adult allergic patients

Affiliations
  • 1Department of Internal Medicine, Yeungnam University College of Medicine, Daegu, Korea. jhchn@med.yu.ac.kr
  • 2Department of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon, Korea.
  • 3Department of Internal Medicine, Dong-A University College of Medicine, Busan, Korea.

Abstract

PURPOSE
This study investigated the differences in the profile of IgE-binding components between Dermatophagoides pteronyssinus (Dp) and Dermatophagoides farina (Df) in respiratory allergic patients sensitized to Dp/Df.
METHODS
Eighteen patients with respiratory allergic diseases having higher levels of serum specific IgE to Df compared to those to Dp (>twice) were enrolled. IgE-immunoblot analysis using Dp and Df extracts were used to compare the IgE binding components. Study subjects were classified into 2 groups according to the results of IgE-immunoblot analysis: 6 subjects having IgE-binding components to group 1 and 2 allergens (group B) and 12 subjects not having them (group A).
RESULTS
Group A subjects were older (47.92±8.51 vs. 35.50±11.10, P=0.039) and males were dominant (75% vs. 0% P=0.009). IgE-immunoblot analysis demonstrated that all the group B subjects had IgE bindings to 2 major components, 14 and 25 kDa, while group A subjects had IgE bindings to high-molecular weight components ranging from 60-98 kDa. The enzyme-linked immunosorbent assay inhibition test showed a significant inhibition with additions of Df, not with Dp in group B subjects. Serum specific IgE levels to Dp and Df were significantly higher in group B than in group A, while its ratio (Df to Dp) was significantly higher in group A. No differences were noted in clinical parameters, total IgE, or eosinophil cationic protein levels.
CONCLUSION
Heterogeneity of IgE binding patterns to Dp and Df extracts was noted according to the ratio of serum specific IgE (Df/Dp).

Keyword

Dermatophagoides pteronyssinus; Dermatophagoides farinae; IgE binding components

MeSH Terms

Adult*
Allergens
Dermatophagoides farinae
Dermatophagoides pteronyssinus
Dust*
Enzyme-Linked Immunosorbent Assay
Eosinophil Cationic Protein
Humans
Immunoglobulin E
Male
Population Characteristics
Pyroglyphidae*
Allergens
Dust
Eosinophil Cationic Protein
Immunoglobulin E

Figure

  • Fig. 1 Comparisons of serum specific IgE binding components by IgE immunoblot using Dermatophagoides pteronyssinus (Dp) and Dermatophagoides farinae (Df). Group A, the subjects not having IgE-binding components to groups 1 and 2 allergens; group B, the subjects having IgE-binding components to groups 1 and 2 allergens; a, Using Dp extracts; b, Using Df extracts; PC, positive control which were derived from the results from the patients with higher serum specific IgE levels to both Dp and Df; NC, negative controls.

  • Fig. 2 IgE enzyme-linked immunosorbent assay inhibition tests with serial additions of Dp, Df in sera from group A (A) and group B (B) subjects. Dp, Dermatophagoides pteronyssinus; Df, Dermatophagoides farina.


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