Allergy Asthma Respir Dis.  2016 May;4(3):167-173. 10.4168/aard.2016.4.3.167.

Stem cell therapy in animal models of allergic airway diseases

Affiliations
  • 1Department of Otorhinolaryngolgy-Head and Neck Surgery, Pusan National University School of Medicine, Busan, Korea. choks@pusan.ac.kr

Abstract

Allergic airway diseases are characterized by T-helper type 2 (Th2)-skewed eosinophilic inflammation, mucus hypersecretion, and airway hyperresponsiveness. The excessive activation of Th2 cells due to insufficient suppression of regulatory T cells (Tregs) is thought to play a major role in the initiation and development of allergic airway disease. Several studies have shown that stem cells provide a significant reduction in allergic airway inflammation and improve lung function in animal models. The immunomodulatory effects of stem cells in allergic airway disease may be mediated by the up-regulation of Tregs and increases in several soluble factors, such as prostaglandin E2, transforming growth factor-β, interleukin-10, and indoleamine 2, 3-dioxygenase. This review examines the current understanding of the immunomodulatory properties of stem cells and its therapeutic implication in allergic airway disease. Furthermore, we will discuss mechanisms by which stem cells inhibit allergic airway inflammation via immunomodulation from a Th2- to a Th1-biased response.

Keyword

Stem cells; Immunosuppression; Allergic rhinitis; Asthma; Regulatory T lymphocytes

MeSH Terms

Animals*
Asthma
Dinoprostone
Eosinophils
Immunomodulation
Immunosuppression
Inflammation
Interleukin-10
Lung
Models, Animal*
Mucus
Rhinitis, Allergic
Stem Cells*
T-Lymphocytes, Regulatory
Th2 Cells
Up-Regulation
Dinoprostone
Interleukin-10

Figure

  • Fig. 1. Adipose tissue-derived stem cells labeled with Cell Tracker CM-Dil (red) were more intensively distributed within the lung of asthmatic mice (A) than control mice (B) (magnification, ×200).

  • Fig. 2. Schematic illustration of the soluble factors for MSC-mediated immunosuppression. TLR, toll-like receptor; MSC, mesenchymal stem cell; DC, dendritic cells; HGF, hepatocyte growth factor; IL, interleukin; PGE2, prostaglandin E2; IDO, indoleamine 2,3-dioxygenase; NO, nitric oxide; PDL-1, programmed death ligand-1; TGF-β1, transforming growth factor-β1; SDF-1, stem cell derived factor 1; VEGF, vascular endothelial growth factor; IL-1RA, interleukin-1 receptor antagonist; TNF-α, tumor necrosis factor-; IFN-, interferon; Ig, immunoglobulin; NK, natural killer; MAC, macrophage. Adapted from Sueblinvong et al. Transl Res 2010;156:188–205.33

  • Fig. 3. Schematic presentation of possible immunomodulatory mechanisms of stem cells in allergic airway diseases. AR, allergic rhinitis; AHR, airway hyperrespon-siveness; IDO, indoleamine 2,3-dioxygenase; IFN-γ, interferon-γ; Ig, immunoglobulin; IL, interleukin; PGE2, prostaglandin E2; TGF-β, transforming growth factor-β; Tregs, regulatory T cells.


Reference

References

1. Uccelli A, Moretta L, Pistoia V. Mesenchymal stem cells in health and disease. Nat Rev Immunol. 2008; 8:726–36.
Article
2. Tuan RS, Boland G, Tuli R. Adult mesenchymal stem cells and cell-based tissue engineering. Arthritis Res Ther. 2003; 5:32–45.
3. Law S, Chaudhuri S. Mesenchymal stem cell and regenerative medicine: regeneration versus immunomodulatory challenges. Am J Stem Cells. 2013; 2:22–38.
4. Delorme B, Charbord P. Culture and characterization of human bone marrow mesenchymal stem cells. Methods Mol Med. 2007; 140:67–81.
Article
5. Liu TM, Martina M, Hutmacher DW, Hui JH, Lee EH, Lim B. Identification of common pathways mediating differentiation of bone marrow- and adipose tissue-derived human mesenchymal stem cells into three mesenchymal lineages. Stem Cells. 2007; 25:750–60.
Article
6. Titorencu I, Jinga V, Constantinescu E, Gafencu A, Ciohodaru C, Mano-lescu I, et al. Proliferation, differentiation and characterization of osteoblasts from human BM mesenchymal cells. Cytotherapy. 2007; 9:682–96.
Article
7. Bhagavati S, Xu W. Isolation and enrichment of skeletal muscle progenitor cells from mouse bone marrow. Biochem Biophys Res Commun. 2004; 318:119–24.
Article
8. Shi M, Liu ZW, Wang FS. Immunomodulatory properties and therapeutic application of mesenchymal stem cells. Clin Exp Immunol. 2011; 164:1–8.
Article
9. Ma S, Xie N, Li W, Yuan B, Shi Y, Wang Y. Immunobiology of mesenchymal stem cells. Cell Death Differ. 2014; 21:216–25.
Article
10. Cho KS, Park HK, Park HY, Jung JS, Jeon SG, Kim YK, et al. IFATS collection: immunomodulatory effects of adipose tissue-derived stem cells in an allergic rhinitis mouse model. Stem Cells. 2009; 27:259–65.
Article
11. Sun YQ, Deng MX, He J, Zeng QX, Wen W, Wong DS, et al. Human pluripotent stem cell-derived mesenchymal stem cells prevent allergic airway inflammation in mice. Stem Cells. 2012; 30:2692–9.
Article
12. Fu QL, Chow YY, Sun SJ, Zeng QX, Li HB, Shi JB, et al. Mesenchymal stem cells derived from human induced pluripotent stem cells modulate T-cell phenotypes in allergic rhinitis. Allergy. 2012; 67:1215–22.
Article
13. Park HK, Cho KS, Park HY, Shin DH, Kim YK, Jung JS, et al. Adipose-derived stromal cells inhibit allergic airway inflammation in mice. Stem Cells Dev. 2010; 19:1811–8.
Article
14. Ge X, Bai C, Yang J, Lou G, Li Q, Chen R. Intratracheal transplantation of bone marrowderived mesenchymal stem cells reduced airway inflammation and upregulated CD4+ CD25+ regulatory T cells in asthmatic mouse. Cell Biol Int. 2013; 37:675–86.
15. Ge X, Bai C, Yang J, Lou G, Li Q, Chen R. Effect of mesenchymal stem cells on inhibiting airway remodeling and airway inflammation in chronic asthma. J Cell Biochem. 2013; 114:1595–605.
Article
16. Boulay ME, Boulet LP. The relationships between atopy, rhinitis and asthma: pathophysiological considerations. Curr Opin Allergy Clin Immunol. 2003; 3:51–5.
Article
17. Togias A. Rhinitis and asthma: evidence for respiratory system integration. J Allergy Clin Immunol. 2003; 111:1171–83.
Article
18. Wilson MS, Taylor MD, Balic A, Finney CA, Lamb JR, Maizels RM. Suppression of allergic airway inflammation by helminth-induced regulatory T cells. J Exp Med. 2005; 202:1199–212.
Article
19. Shi HZ, Qin XJ. CD4CD25 regulatory T lymphocytes in allergy and asthma. Allergy. 2005; 60:986–95.
Article
20. Jaffar Z, Sivakuru T, Roberts K. CD4+CD25+ T cells regulate airway eosinophilic inflammation by modulating the Th2 cell phenotype. J Immunol. 2004; 172:3842–9.
Article
21. Puissant B, Barreau C, Bourin P, Clavel C, Corre J, Bousquet C, et al. Immunomodulatory effect of human adipose tissue-derived adult stem cells: comparison with bone marrow mesenchymal stem cells. Br J Haematol. 2005; 129:118–29.
Article
22. Yanez R, Lamana ML, García-Castro J, Colmenero I, Ramirez M, Bueren JA. Adipose tissue-derived mesenchymal stem cells have in vivo immunosuppressive properties applicable for the control of the graft-versus-host disease. Stem Cells. 2006; 24:2582–91.
Article
23. Glennie S, Soeiro I, Dyson PJ, Lam EW, Dazzi F. Bone marrow mesenchymal stem cells induce division arrest anergy of activated T cells. Blood. 2005; 105:2821–7.
Article
24. Corcione A, Benvenuto F, Ferretti E, Giunti D, Cappiello V, Cazzanti F, et al. Human mesenchymal stem cells modulate B-cell functions. Blood. 2006; 107:367–72.
Article
25. Ramasamy R, Fazekasova H, Lam EW, Soeiro I, Lombardi G, Dazzi F. Mesenchymal stem cells inhibit dendritic cell differentiation and function by preventing entry into the cell cycle. Transplantation. 2007; 83:71–6.
Article
26. English K, Barry FP, Mahon BP. Murine mesenchymal stem cells suppress dendritic cell migration, maturation and antigen presentation. Immunol Lett. 2008; 115:50–8.
Article
27. Cohn L, Elias JA, Chupp GL. Asthma: mechanisms of disease persistence and progression. Annu Rev Immunol. 2004; 22:789–815.
Article
28. Aggarwal S, Pittenger MF. Human mesenchymal stem cells modulate allogeneic immune cell responses. Blood. 2005; 105:1815–22.
Article
29. Beyth S, Borovsky Z, Mevorach D, Liebergall M, Gazit Z, Aslan H, et al. Human mesenchymal stem cells alter antigen-presenting cell maturation and induce T-cell unresponsiveness. Blood. 2005; 105:2214–9.
Article
30. Sato K, Ozaki K, Oh I, Meguro A, Hatanaka K, Nagai T, et al. Nitric oxide plays a critical role in suppression of T-cell proliferation by mesenchymal stem cells. Blood. 2007; 109:228–34.
Article
31. Cui L, Yin S, Liu W, Li N, Zhang W, Cao Y. Expanded adipose-derived stem cells suppress mixed lymphocyte reaction by secretion of prostaglandin E2. Tissue Eng. 2007; 13:1185–95.
Article
32. Cho KS, Roh HJ. Immunomodulatory effects of adipose-derived stem cells in airway allergic diseases. Curr Stem Cell Res Ther. 2010; 5:111–5.
Article
33. Sueblinvong V, Weiss DJ. Stem cells and cell therapy approaches in lung biology and diseases. Transl Res. 2010; 156:188–205.
Article
34. Cho KS, Park MK, Kang SA, Park HY, Hong SL, Park HK, et al. Adipose-derived stem cells ameliorate allergic airway inflammation by inducing regulatory T cells in a mouse model of asthma. Mediators Inflamm. 2014; 2014:436476.
Article
35. English K, Ryan JM, Tobin L, Murphy MJ, Barry FP, Mahon BP. Cell contact, prostaglandin E(2) and transforming growth factor beta 1 play non-redundant roles in human mesenchymal stem cell induction of CD4+ CD25 (High) forkhead box P3+ regulatory T cells. Clin Exp Immunol. 2009; 156:149–60.
36. Nemeth K, Keane-Myers A, Brown JM, Metcalfe DD, Gorham JD, Bun-doc VG, et al. Bone marrow stromal cells use TGF-beta to suppress allergic responses in a mouse model of ragweed-induced asthma. Proc Natl Acad Sci U S A. 2010; 107:5652–7.
37. Cho KS, Lee JH, Park MK, Park HK, Yu HS, Roh HJ. Prostaglandin E2 and Transforming Growth Factor-β Play a Critical Role in Suppression of Allergic Airway Inflammation by Adipose-Derived Stem Cells. PLoS One. 2015; 10:e0131813.
Article
38. Kupcova Skalnikova H. Proteomic techniques for characterisation of mesenchymal stem cell secretome. Biochimie. 2013; 95:2196–211.
Article
39. Kim HO, Choi SM, Kim HS. Mesenchymal stem cell-derived secretome and microvesicles as a cell-free therapeutics for neurodegenerative disorders. Tissue Eng Regen Med. 2013; 10:93–101.
Article
Full Text Links
  • AARD
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr