Korean J Blood Transfus.  2016 Apr;27(1):79-83. 10.17945/kjbt.2016.27.1.79.

A Case of Hemolytic Transfusion Reaction Attributable to Anti-Jk(b) Abolished in the Enzyme Phase Reaction

• Song SA
• Oh SH
• Lee JY
• Sung SK
• Shin JH
• Kim HR
• Jun KR
• Lee JN

• KMID: 2163123
• DOI: http://doi.org/10.17945/kjbt.2016.27.1.79

Abstract

We report a case of an intravascular hemolytic reaction attributable to anti-Jk(b) antibodies that were not detected using an enzyme phase antibody identification test. A 61-year-old male who had received two units of red blood cells was admitted to the emergency room because his urine was dark. LISS/Coombs gel column agglutination tests suggested the presence of anti-Jk(b) and anti-E antibodies. However, his serum was negative for the Jk(b) antigen when an enzyme phase test was performed. A positive reaction was evident, however, when EDTA-treated plasma was tested; this excluded any possible complement-mediated reaction. The patient was diagnosed with an intravascular hemolytic transfusion reaction, caused by anti-Jk(b), and was later discharged without specific complications after receiving antigen-negative blood transfusions.

Keyword

Kidd Blood-Group System; Enzyme; EDTA; Hemolytic transfusion reaction

MeSH Terms

Agglutination Tests
Antibodies
Blood Group Incompatibility*
Blood Transfusion
Edetic Acid
Emergency Service, Hospital
Erythrocytes
Humans
Kidd Blood-Group System
Male
Middle Aged
Plasma
Antibodies
Edetic Acid
Kidd Blood-Group System