J Korean Med Sci.  2010 Oct;25(10):1418-1426. 10.3346/jkms.2010.25.10.1418.

Stemness Evaluation of Mesenchymal Stem Cells from Placentas According to Developmental Stage: Comparison to Those from Adult Bone Marrow

• Sung HJ
• Hong SC
• Yoo JH
• Oh JH
• Shin HJ
• Choi IY
• Ahn KH
• Kim SH
• Park Y
• Kim BS

• KMID: 2157843
• DOI: http://doi.org/10.3346/jkms.2010.25.10.1418

Abstract

This study was done to evaluate the stemness of human mesenchymal stem cells (hMSCs) derived from placenta according to the development stage and to compare the results to those from adult bone marrow (BM). Based on the source of hMSCs, three groups were defined: group I included term placentas, group II included first-trimester placentas, and group III included adult BM samples. The stemness was evaluated by the proliferation capacity, immunophenotypic expression, mesoderm differentiation, expression of pluripotency markers including telomerase activity. The cumulative population doubling, indicating the proliferation capacity, was significantly higher in group II (P<0.001, 31.7+/-5.8 vs. 15.7+/-6.2 with group I, 9.2+/-4.9 with group III). The pattern of immunophenotypic expression and mesoderm differentiation into adipocytes and osteocytes were similar in all three groups. The expression of pluripotency markers including ALP, SSEA-4, TRA-1-60, TRA-1-81, Oct-4, and telomerase were strongly positive in group II, but very faint positive in the other groups. In conclusions, hMSCs from placentas have different characteristics according to their developmental stage and express mesenchymal stemness potentials similar to those from adult human BMs.

Keyword

Stemness; Placenta; Bone Marrow; Mesenchymal Stem Cells

MeSH Terms

Antigens, Surface/metabolism
Bone Marrow Cells/*cytology/metabolism
Cell Proliferation
Female
Humans
Immunophenotyping
Mesenchymal Stem Cells/*cytology/metabolism
Mesoderm/cytology
Octamer Transcription Factor-3/metabolism
Placenta/*cytology/growth & development
Pregnancy
Pregnancy Trimester, First
Proteoglycans/metabolism
Stage-Specific Embryonic Antigens/metabolism
Telomerase/metabolism